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TISSUE PROCESSING Notes - National Institute of Open …

The technique of getting fixed tissues into paraffin is called tissue processing. This describes the steps required to take animal and human tissues from fixation to the state where it is completely infiltrated with a suitable wax i.e. paraffin wax and can be embedded and ready for section cutting on microtome. OBJECTIVES

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PETROCHEMICALS Notes

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SHOOT SYSTEM - National Institute of Open Schooling

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IHC-PARAFFIN PROTOCOL (IHC-P) - Abcam

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IHC-P refers to the staining of tissues that have been fixed (usually in neutral buffered formalin) and then embedded in paraffin before being sectioned. The basic steps of the IHC-P protocol are as follows: 1. Fixing and embedding the tissue 2. Cutting and mounting the section 3. Deparaffinizing and rehydrating the section 4. Antigen retrieval 5.

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