Transcription of Trichoderma asperellum, Identified as a Novel …
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Trichoderma asperellum, Identified as a Novel …
Abstract Twenty isolates of biocontrol Trichoderma species were isolated from rhizosphere soils of different host plants in various locations of tamil nadu , India. Of which, TTH1 exhibited better inhibition ( ) of mycelial growth of the pathogen, S. rolfsii followed by TV 1 (60%) and TED 1 (60%) under in vitro conditions. These three isolates were further tested for their chitinase activity on agarose gel amended with chitin. TTH 1 produced more integrated dense value (IDV) of 105015 on agarose gel followed by TV 1 (96385) and TED 1 (77487) isolates. It indicated that TTH 1 produced more chitinase to degrade the cell wall of pathogen while tested in dual plate technique. The ITS1 to ITS2 region of TTH 1 isolate was amplified and compared with nucleotide sequences from the GenBank database after sequencing.
Tamil Nadu Agricultural University, Coimbatore, India. (e-mail: raguchander@rediffmail.com) Samiyappan Ramasamy Former …
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