Transcription of Troubleshooting and Diagnostics Tips and Tricks
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Troubleshooting and DiagnosticsTips and Tricks 2013 Waters Corporation1 Cerdanyola del Vall s, Enero 2014 Tips and Tricks Troubleshooting and diagnosticsTroubleshooting and Diagnostics Troubleshooting System Pressure Problems Incorrect Retention time Loss of precision Carryover/Contamination 2013 Waters Corporation2 Carryover/Contamination Split and Distorted Peaks Baseline NoiseTroubleshooting and diagnosticsTroubleshooting and Diagnostics Troubleshooting System Pressure Problems Incorrect Retention time Loss of precision Carryover/Contamination 2013 Waters Corporation3 Carryover/Contamination Split and Distorted Peaks Baseline NoiseSystem Pressure problemsSystem Pressure problems To identify a pressure change from normal operation, create a pressure reference point System Pressure is affected by the column, mobile phase, flow rate, temperature and can vary greatly with different 2013 Waters Corporation4flow rate, temperature and can vary greatly with different methodsSystem Pressure problemsSystem Pressure problems Erratic flow rates/pressure pulsations Overpressure No or low pressure 2013 Waters Corporation5
Inject a pure standard Inject a sample with matrix. – If there are other peaks they came from the matrix. Inject a blank of the sample diluent alone. ©2013 Waters Corporation 30 – Is it different from a water blank? Inject volume zero
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