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Understanding (and Creating) Polar Retention Using ...
2003 Waters CorporationUnderstanding (and creating ) Polar Retention Using Reversed-Phase hplc and hydrophilic Interaction ChromatographyPittcon March 2003 2003 Waters CorporationIntroduction Introduction The Problem Background hydrophilic Interaction chromatography Reversed-Phase hplc for Polar Molecules Applications Summary 2003 Waters CorporationIntroduction - The Problem Conventional hplc columns often do not provide adequate Retention and separation of highly Polar compounds Drug Discovery Gradient - High-throughput screening (HTS) does not work for Polar compounds Elute un-retained in the void volume Co-elute at the beginning of the run if retained at all Method Development Isocratic- Many RP columns cannot be run under the 100% aqueous conditions necessary for Polar compound Retention columns dewet (also termed -hydrophobic collapse) 2003 Waters CorporationBackground Introduction Background Polar molecules Chromatographic methods for retaining Polar compounds hydrophilic Interaction chromatography Reversed-Phase hplc for Polar Molecules Applications Summary 2003 Waters CorporationWhat is a Polar Molecule?
©2003 Waters Corporation Understanding (and Creating) Polar Retention Using Reversed-Phase HPLC and Hydrophilic Interaction Chromatography Pittcon
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