Transcription of Version 12 May 2013 - BSAC
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Version 12 May 2013 - BSAC
BSAC Methods for Antimicrobial Susceptibility Testing Version 12 May 2013 All enquiries to: Mandy Wootton Email: Telephone: +44 (0) 2920 746581 Version 12 January 2013 2 Contents Page Working Party members 5 Abstract 6 Preface 8 Disc Diffusion Method for Antimicrobial Susceptibility Testing 1. Preparation of plates 10 2. Selection of control organisms 11 Table 2 a Control strains to monitor test performance of antimicrobial susceptibility testing 12 2b Control strains used to confirm that the method will detect resistance 12 3. Preparation of inoculum 12 Comparison with McFarland standard 13 Preparation of the McFarland standard 13 Inoculum preparation by the growth method 13 Inoculum preparation by the direct colony suspension method 13 Adjustment of the organism suspension to the density of the McFarland standard 13 Dilution of suspension equivalent to McFarland standard in distilled water before inoculation 13 Photometric standardisation of turbidity of suspension 14 Direct susceptibility testing of urines and blood cultures 15 4.
BSAC Methods for Antimicrobial Susceptibility Testing Version 12 May 2013 All enquiries to: Mandy Wootton Email: Mandy.Wootton@wales.nhs.uk
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