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223 #223/AP08 Transformation with Green Fluorescent Protein (GFP)Experiment Objective:Students explore the biological process of bacterial transformation using and plasmid DNA. At the end of the activity, students will have experience observing and analyzing acquired traits (ampicillin resistance and fl uorescence) as exhibited by transformed bacterial page 3 for storage LITERATURE Please refer to included weblink for correct version. Page Experiment Components 3 Experiment Requirements 4 Background Information 5 Experiment Procedures Experiment Overview 8 Laboratory Safety 10 Transformation of with Green Fluorescent Protein 11 Experiment Results and Analysis 13 Study Questions 14 Instructor's Guide Notes to the Instructor 15 Pre-Lab Preparations 16 Experiment Results and Analysis 20 Study Questions and Answers 21 Appendices 22 A EDVOTEK Troubleshooting Guide - Transformation 23 Safety Data Sheets can be found on our website: of ContentsTransformation with Green Fluorescent Protein (GFP)EDVO-Kit #223 Fax of any part of this document is permitted for non-profi t educational purposes only.
DNA CAN BE TRANSFERRED BETWEEN BACTERIA In nature, DNA is transferred between bacteria using two main meth-ods— transformation and conjugation.
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