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Viral Replication: Basic Concepts - Columbia University

1 Viral ReplicationScott M. Hammer, Replication: Basic Concepts Viruses are obligate intracellular parasites Viruses carry their genome (RNA or DNA) and sometimes functional proteins required for early steps in replication cycle Viruses depend on host cell machinery to complete replication cycle and must commandeer that machinery to successfully replicate2 Viral Replication: Basic Concepts Replication cycle produces-Functional RNA s and proteins-Genomic RNA or DNA and structural proteins 100 s-1,000 s new particles produced by each cycle-Referred to as burst size-Many are defective-End of eclipse phase Replication may be cytolytic or non-cytolyticSteps in Viral Replication.

Native Form Fusion peptide HR1 HR2 Fusion Intermediate T20----- - ----- -- - - - - - - - - -----“Ensnared” Transition State Intermediate Conformation Fusion Blockade Membrane Fusion Core Structure T1249 Mechanism of T20/T1249 Mediated Fusion Inhibition Modified from Weissenhorn et al., Nature 387, 426-430 (1997) and Furuta et al., Nature ...

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