Transcription of Yeast Protocols Handbook
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Yeast Protocols Handbook
Yeast Protocols Handbook FOR RESEARCH USE ONLYPT3024-1 (PR742227)Published 11 February 2008 Yeast Protocols Handbook Clontech Laboratories, Inc. Protocol No. PT3024-1 2 Version No. PR742227 I. Introduction 4 II. Introduction to Yeast Promoters 5 III. Culturing and Handling Yeast 10 IV. Preparation of Yeast Protein Extracts 12 A. General Information 12 B. Preparation of Yeast Cultures for Protein Extraction 12 C. Preparation of Protein Extracts: Urea/SDS Method 13 D. Preparation of Protein Extracts: TCA Method 15 E. Troubleshooting 17 V. Yeast Transformation Procedures 18 A. General Information 18 B. Reagents and Materials Required 19 C. Tips for a Successful Transformation 20 D. Integrating Plasmids into the Yeast Genome 20 E. Small-scale LiAc Yeast Transformation Procedure 20 F. Troubleshooting Yeast Transformation 22 VI. - and -Galactosidase Assays 23 A. General Information 23 B.
In vivo Plate Assay Using X-gal in the Medium 25 ... Sequence of GAL4 DNA-BD recognition sites in the GAL1, GAL2, MEL1 UASs and the UAS G 17-mer 6 Figure 2. Urea/SDS protein extraction method 14 ... obtain a license from the Research Foundation of the State University of New York before using them. Clontech is required
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