Iodine Affinity
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OCULAR and STAGE MICROMETERS - Biology Department
fire.biol.wwu.eduThe crystal violet treatment must precede iodine treatment. Iodine acts as a mordant, i.e., it increases the affinity of the cells for the crystal violet. Iodine alone has no bacterial staining capabilities. 2. Decolorization must be short and precise. Too long an exposure to 95% alcohol will decolorize Gram-positive as well as Gram-negative cells.
STAINING OF BACTERIAL CELLS Objective Introduction
www.sas.upenn.edudye, the chromophore can be either positively charged (cationic) and have an affinity for negative ions or negatively charged (anionic) with an affinity for positive ions. Bacteria carry a net negative charge at pH 7. Therefore, cationic dyes such as methylene blue, basic fuchsin, or ... with the iodine mordant after primary staining, they too ...
Armour® Thyroid (thyroid tablets, USP)
media.allergan.comThe higher affinity of levothyroxine (T 4) for both TBg and TBPA as compared to liothyronine (T 3) partially explains the higher serum levels and longer half-life of the former hormone. Both protein-bound hormones exist in reverse equilibrium with ... or inorganic iodine.
Bacterial Classification, Structure and Function
www.columbia.educrystal violet, iodine, then destained with alcohol and counter-stained with safranin. Gram positive bacteria stain blue-purple and Gram negative bacteria stain red. The difference between the two groups is believed to be due to a much larger peptidoglycan (cell wall) in Gram positives. As a result the iodine and crystal violet precipitate in the