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Protocol:Real -time RTPCRassays forthe detection ...

Protocol:Real -time RTPCRassays forthe detection ...

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RNA extracted from 100 µl of original sample, is eluted in 100 µl of elution buffer. 2 MIX PREPARATION FOR ALL SEPARATE PRIMER/PROBE COMBINATIONS All primers and probes described below were validated under the following conditions. RT-PCR Mix kit:

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