Example: stock market
Measurement Using The Dye Dilution
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Measuring Cell Viability / Cytotoxicity
www.dojindo.eu.comMeasurement Wavelength: 450nm Fig. 4 Color development using HL60 cells Media: RPMI1640 (10% FBS) Incubation: 37 oC, 3 hr, 5% CO2 incubator Measurement Wavelength: 450nm Fig. 5 Correlation between CCK-8 assay and [3H]-Thymidine Uptake assay Cell Counting Kit-8 assay Serial Dilution Procedure Using an 8 channel multi-pipette, add 100 µl of media
Calculate dye:protein (F/P) molar ratios
tools.thermofisher.com) for the dye molecule • CF = Correction factor; adjusts for the amount of absorbance at 280 nm caused by the dye (see Table 1) • Dilution factor = the extent (if any) to which the protein:dye sample was diluted for absorbance measurement . dilution