Analysis of Relative Gene Expression Data Using …
ANALYSIS OF REAL-TIME PCR DATA 403 1. THE 22DDCT METHOD or X N 3 (1 1 E)DCT 5 K, [6] 1.1. Derivation of the 22DDCT Method The equation that describes the exponential amplifi- where X N is equal to the normalized amount of target cation of PCR is (X0/R 0) and DC T is equal to the difference in threshold cycles for target and …
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Principle and applications of digital PCR - GENE
www.gene-quantification.netDigital PCR www.future-drugs.com 43 To address whether digital PCR is useful for mutation detec-tion in cancer, Vogelstein and Kinzler have analyzed the DNA
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