Buffer Formulations - Bio-Rad
This is an excerpt from Bio-Rad’s comprehensive Electrophoresis Guide (Bulletin 6640). Running Buffers Buffer Components 10× SDS-PAGE (1 L) (cata log #161-0732) 250 mM Tris, 1.92 M glycine, 1% SDS, pH 8.3 Tris base 30.30 g Glycine 144.10 g SDS 10.00 g diH 2O to 1 L Do not adjust the pH (~pH 8.3). ...
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