Direct ELISA protocol - Abcam
Blocking solution Commonly used blocking agents are 1% BSA, serum, non-fat dry milk, casein, gelatin in PBS. Wash solution Usually PBS or Tris-buffered saline (pH 7.4) with detergent such as 0.05% (v/v) Tween20 (TBST). Antibody dilution buffer Primary and secondary antibody should be diluted in 1x blocking solution to reduce non-specific binding.
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