Flash Column Chromatography Guide - MIT OpenCourseWare
elution. This simply means that you increase the polarity of the solvent running through the column (eluent) throughout the course of the purification. This technique will be described in more detail later in the handout, but for the TLC analysis you should determine which solvent systems put the different spots in the 0.2 to 0.3 Rf range. ...
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