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GRAM STAIN PROTOCOL

GRAM STAIN PROTOCOL

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C. Staining procedures a. Hucker’s modification i. Flood the fixed smear with the crystal violet solution; allow the stain to remain for 30 s. ii. Decant crystal violet, and rinse slide gently with running water. Caution: Excessive rinsing in this step could cause crystal violet to be washed from gram …

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