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HPLC METHOD DEVELOPMENT AND VALIDATION: AN …

HPLC METHOD DEVELOPMENT AND VALIDATION: AN …

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accuracy, precision, and convenience of the final method [8]. Step 2 - Selection of initial conditions This step determines the optimum conditions to adequately retain all analytes; that is, ensures no analyte has a capacity factor of less than 0.5 (poor retention could result in peak overlapping) and no analyte has a capacity

  Optimum, Convenience

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