HPLC Troubleshooting Guide
2. Add basic mobile phase additive (eg. TEA) – not needed with ultra-high purity phases Chelation with metal ions in stationary phase As above Wrong mobile phase pH 1. Decrease mobile phase pH to suppress silanol ionisation (see 1.2) 2. Increase buffer concentration (see 1.2) Blocked frit 1. Reverse flush the column (see 5.2) 2.
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