Immunohistochemistry (IHC)
The label, either fluorescent or enzymatic, is used to visualize the antigen-antibody complex. In this multi-step application, staining may be influenced by multiple variables and requires the careful optimization of new assays for each target antigen. For example, when investigating a high abundance protein in formaldehyde-fixed tissue, the IHC
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Immunocytochemistry (ICC)
images.novusbio.comamino acid containing components including flavin coenzymes (FAD, FMN) and pyridine nucleotides (NADH). Refer to Figure 4A for an example of autofluorescence emitted by Lipofuscin, a pigment that accumulates with age in many tissue types. Lipofuscin has autofluorescence properties that overlap with the emission spectra of commonly used ...
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