iScript cDNA Synthesis Kit - Bio-Rad
RNA template (100 fg–1 μg total RNA)* Variable Total volume 20 * When using larger amounts of input RNA (>1 μg), the reaction should be scaled up (for example, 40 μl reaction for 2 μg, or 100 μl reaction for 5 μg) to ensure optimum synthesis efficiency. Reaction Protocol Incubate the complete reaction mix in a thermal cycler using
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