Making Electrocompetent Cells
3. Add 10g Bacto Yeast Extract. 4. Add 5g NaCl. 5. Adjust pH to 7.0 with 5N NaOH. 6. Adjust to 1L with distilled H2O. 7. Sterilize by autoclaving on LIQUID cycle. LB (Luria-Bertani) Broth Recipe 10 grams tryptone 5 grams of yeast extract 10 grams of NaCl (NOTE: A premixed powder is also available from Fisher cat# BP1426) 1. Dissolve in 1 L of ...
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