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Next-Generation DNA Sequencing Methods

Next-Generation DNA Sequencing Methods

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lapping set or tiling path through each human chromosome as determined by physical map-ping(31).InBAC-basedsequencing,eachBAC clone is amplified in bacterial culture, isolated inlargequantities,andshearedtoproducesize-selected pieces of approximately 2−3 kb. These pieces are subcloned into plasmid vectors, am-

  Lapping

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