QUANTITATIVE RT-PCR PROTOCOL (SYBR Green I)
Apr 01, 2007 · Quantitative RT-PCR Protocol (SYBR Green I) 7 d. Calculate the fold-change of each gene for the two samples and normalize them by using the spiking human gene as reference Ratio= E TARGET C T TARGET( )()Control sample E REF C T REF( )()control sample Note: “control-sample” could be “sample 1-sample 2”.
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