RT-PCR: Two-Step Protocol - MIT OpenCourseWare
Reverse Transcriptase 100 U/µl 100U 1 µl 0l µ Nuclease-free water x µl (to total of 20 µl) x µl (to total of 20 µl) 2) Mix gently, spin briefly. 3) Incubate in the thermacycler at: a. 44°C for 1 hr. b. 92°C for 10 min to inactivate the reverse transcriptase. 4) Store reaction at …
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