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Transfer Buffer Formulations - Bio-Rad Laboratories

Transfer Buffer Formulations - Bio-Rad Laboratories

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10 mM NaHCO 3, 3 mM Na 2 CO 3, 20% methanol (pH 9.9) NaHCO 3 0.84 g Na 2 CO 3 (anhydrous) 0.318 g diH 2 O 500 ml Methanol 200 ml Adjust volume to 1 L with diH 2 O. 0.7% Acetic Acid Add 7 ml glacial acetic acid to 993 ml diH 2 O. Protocol Transfer Buffer Formulations Bulletin 6211 TIPS Use only high-quality, analytical grade methanol.

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