TRIzol Reagent User Guide - Pub. no. MAN0001271 - Rev. A
Isolate DNA Isolate DNA from the interphase and the lower phenol-chloroform phase saved from “Lyse samples and separate phases“ on page 2. a. Remove any remaining aqueous phase overlying the interphase. This is critical for the quality of the isolated DNA. b. Add 0.3 mL of 100% ethanol per 1 mL of TRIzol™ Reagent used for lysis.
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