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DNA 抽出 - UMIN

QCWS DNA . QCWS . DNA . 29 .. QCWS . HLA . QCWS DNA .. QCWS DNA .. QIAamp DNA Blood Mini Kit .. 1.. 1.. 1. DNA .. 2. LETFLEX Genomic DNA Purification kit 100 .. 4.. 4.. 5. DNA .. 5. QuickGene DNA whole blood kit S(DB-S) .. 8.. 8.. 8. DNA .. 9.. 11. DNA . QIAamp DNA Blood Mini kit .. QIAamp DNA Blood Mini Kit (QIAGEN/Cat No. 51104 51106). 96 100% .. (1) 56 . (2) 15 25 .. QIAamp DNA Blood Mini Kit (QIAGEN/Cat No. 51104 51106).. 51104 (50 ) 51106 (250 ). Buffer AL 12 mL 54 mL. Buffer AW1 19 mL 95 mL. Buffer AW2 13 mL 66 mL. Buffer AE 12 mL 60 mL. QIAGEN Protease 1 vial 1 vial Protease Solvent mL mL. QIAamp Spin Columns 50 250 . Collection tubes (2 ml) 150 750 . (1) Buffer AW1 125 ml 96 100% 220 ml .. 1/13. DNA . (2) Buffer AW2 160 ml 96 100% 226 ml .. (3) QIAGEN Protease protease solvent ml ml . QIAamp DNA Blood Mini Kit (50)(51104) ml protease solvent QIAamp DNA. Blood Mini Kit (250)(51106) ml protease solvent . (4) Buffer AL 56.

2．JETFLEX Genomic DNA Purification Kit 100 2．1 検査機器 の準備 2.1.1 機器.器具の準備 機器 ボルテックスミキサー ヒートブロックまたはウーターバス 遠心器 2000g以上または、12000g以上遠心出来るもの 分光光度計 （260nmと280nm）

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Transcription of DNA 抽出 - UMIN

1 QCWS DNA . QCWS . DNA . 29 .. QCWS . HLA . QCWS DNA .. QCWS DNA .. QIAamp DNA Blood Mini Kit .. 1.. 1.. 1. DNA .. 2. LETFLEX Genomic DNA Purification kit 100 .. 4.. 4.. 5. DNA .. 5. QuickGene DNA whole blood kit S(DB-S) .. 8.. 8.. 8. DNA .. 9.. 11. DNA . QIAamp DNA Blood Mini kit .. QIAamp DNA Blood Mini Kit (QIAGEN/Cat No. 51104 51106). 96 100% .. (1) 56 . (2) 15 25 .. QIAamp DNA Blood Mini Kit (QIAGEN/Cat No. 51104 51106).. 51104 (50 ) 51106 (250 ). Buffer AL 12 mL 54 mL. Buffer AW1 19 mL 95 mL. Buffer AW2 13 mL 66 mL. Buffer AE 12 mL 60 mL. QIAGEN Protease 1 vial 1 vial Protease Solvent mL mL. QIAamp Spin Columns 50 250 . Collection tubes (2 ml) 150 750 . (1) Buffer AW1 125 ml 96 100% 220 ml .. 1/13. DNA . (2) Buffer AW2 160 ml 96 100% 226 ml .. (3) QIAGEN Protease protease solvent ml ml . QIAamp DNA Blood Mini Kit (50)(51104) ml protease solvent QIAamp DNA. Blood Mini Kit (250)(51106) ml protease solvent . (4) Buffer AL 56.

2 DNA . DNA . (1) ml QIAGEN Protease20 L . (2) 200 L . 200 L PBS 200 L . EDTA . PCR .. (3) Buffer AL200 L . QIAGEN Protease Buffer AL . (4) 15 . (5) 56 10 . (6) 96 100 200 L 15 .. (7) QIAamp Mini Spin Column 2 ml (6) . (8) 6,000 x g 8,000 rpm 1 . 2/13. DNA . (9) QIAamp Mini Spin Column 2 ml . (10) Buffer AW1 500 L (9) Column . (11) 6,000 x g 8,000 rpm 1 . (12) QIAamp Mini Spin Column 2 ml . (13) Buffer AW2500 L Column . (14) 20,000 x g 14,000 rpm 3 . (15) QIAamp Mini Spin Column 2 ml . (16) 20,000 x g 14,000 rpm 1 Buffer .. (17) QIAamp Mini Spin Column ml . (18) 200 L Buffer AE . (19) 15 25 5 . (20) 6,000 x g 8,000 rpm 1 DNA . (21) ml DNA . 200 L 5 x 106 /ml 200 L . DNA 6 g 30 ng/ L . DNA OD260/280 . (22) Buffer AE 20 ng/ L DNA . I 20ng/ L 4 L II 2 L . 3/13. DNA . jetflex Genomic DNA Purification Kit 100.. 2000 12000 . 260nm 280nm .. 300 L L . 300 L 15 L,50 L.. 100 . jetflex Genomic DNA Purification Kit 100 .. Kit.

3 100 . RBC 33 mL. CLB 33 mL. PPT 17 mL. Pellet Compactor 8 mL. DNA TE 22 mL. Proteinase K * 2 x 21 mg RNase 4 mg/mL 1 mL. *Proteinase K 21mg mL 20 mg/mL .. -20 . *RNase . Kit .. 70% . 4/13. DNA .. PCR .. DNA DNase . DNA . DNA . DNA .. DNA .. (1) RBC . RBC . 300 uL 300 uL mL . 2 mL 2 mL 15 mL . (2) . (3) .. 2000 g 10 . 12000 g 30 . (4) . (5) CBL . 5 10 .. 37 . Protainase K 20mg mL . 58 1 . RNA R ase 37 5 . DNA . (1) PPTB attfa 20 . PPT . 300 uL 150 L. 2 mL 1 mL. 5/13. DNA . (2) .. 2000 g 10 . 12000 g 3 .. Pellet Compactor . 1 50 L Pellet Compactor . 2 .. 1 5 . 2 . (3) . 3 .. 300 uL 900 uL. 2 mL 6 mL. DNA . DNA .. (4) .. 2000 g 10 . 12000 g 3 . (5) .. (6) 70 DNA . 70% . 300 uL 1 mL. 2 mL 6 mL. (7) .. 2000 g 5 . 12000 g 1 . 6/13. DNA . (8) .. (9) DNA DNA . TE DNA . DNA . TE buffer DNA *. 300 L 150 L 20 70 g/uL. 2 mL 400 L 50 175 g/uL. DNA . A260 A280 DNA .. DNA . 100 L DNA 500 L CLB .. 300 L PPT 20 .. 7/13. DNA . QuickGene DNA whole blood kit S(DB-S).

4 DNA OD260/280 .. 99 .. PBS .. (1) QuickGene DNA whole blood kit S(DB-S).. Protease( ) EDB. Lysis Buffer LDB. 15 28 . Wash Buffer WDB. EDB 4 2 . Elution Buffer CDB . Cartridges CA EDB -20 . Collection Tubes CT .. Caps CAP. 8/13. DNA . Waste Tubes WT.. (1) EDB L . 4 2 -20 .. (2) LDB 37 .. (3) WDB 160 L 99 . ethanol added? .. DNA .. (1) 56 . (2) EDB 30 L L .. (3) 200 L . EDTA 2Na EDTA 2K . Buffy coat . 200 L PBS 200 L .. (4) LDB250 L . 5 EDB . LDB EDB LDB .. (5) 15 . 2,500rpm . (6) 56 2 5 . 9/13. DNA . (7) 99 .. QG-810/800 . (1) DNA WHOLE BLOOD . (2) QG-810/800 CT WT .. (3) WDB CDB QG-810/800 . (4) . (5) DISCHARGE .. (6) CA CA .. (7) START .. (8) . CT . QG-810:- QG-800:X . (9) WT CA .. QG-Mini80 . (1) CA CT WT . (2) W CA . 10/13. DNA . CA . (3) WDB750 L CA CA .. (4) . 3 2 . (5) E CDB . CDB 200 L .. (6) CA .. (7) CT CAP . (8) WT .. (1) 260n 260/280n . (2) . * QG-810/800 ** QG-Mini80 .. DNA . DNA .. EDB 30 .. EDB . EDB LDB.

5 200 L . 2 106 PBS. 2 106 . 11/13. DNA .. LDB .. CA .. CDB ** CDB 50 L . CA .. ** CA .. CA ** . CDB CDB . WDB * QG-810/800 1 WDB .. DNA .. * QG-810/800 .. CA . 5 106 .. 2 106 PBS. 2 106 .. LDB .. 37 .. CDB CDB .. * . CDB ** . 12/13. DNA . CDB . ** E .. CA CA .. ** . 13/13.

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