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PHOSPHATE BUFFERED SALINE (PBS) - biologija.org

LLLLABORATORY FOR EXPERIABORATORY FOR EXPERIABORATORY FOR EXPERIABORATORY FOR EXPERIMENTAL BOTANYMENTAL BOTANYMENTAL BOTANYMENTAL BOTANY UNIVERSITY OF LJUBLJANA VECNA POT 111 BIOTECHNICAL FACULTY SI-1000 LJUBLJANA, SLOVENIA DEPARTMENT OF BIOLOGY contact email: Last change: 2007-02-01 1/1 PHOSPHATE BUFFERED SALINE (PBS) 10x PBS (sodium-potassium combo), 1 L: 2 g KCl g KH2PO4 80 g NaCl g Na2 HPO4 note: if Na2 HPO4 (7H2O), use , if Na2 HPO4 (12H2O), use Dissolve everything in approximately 800 ml of dH2O, then fill up to 1000 ml. Autoclave and store at room temperature. 10x PBS, pH 7 4 (sodium), 100 mL: 7,6 g NaCl (Mw g/mol, M = 1,3 mol/L) 0,99 g Na2 HPO4 (Mw g/mol, M = 0,07 mol/L) 0,41 g NaH2PO4 x H2O (Mw g/mol, M = 0,03 mol/L) Adjust pH with 1 M NaOH or 1 M HCl. Fill up to 100 ml. Autoclave and store at room temperature. Calculation of Mw: CHEMIX ( )

LLLABORATORY FOR EXPERIMENTAL BOTANYABORATORY FOR EXPERI MENTAL BOTANY UNIVERSITY OF LJUBLJANA VECNA POT 111 BIOTECHNICAL FACULTY SI-1000 LJUBLJANA, SLOVENIA ...

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Transcription of PHOSPHATE BUFFERED SALINE (PBS) - biologija.org

1 LLLLABORATORY FOR EXPERIABORATORY FOR EXPERIABORATORY FOR EXPERIABORATORY FOR EXPERIMENTAL BOTANYMENTAL BOTANYMENTAL BOTANYMENTAL BOTANY UNIVERSITY OF LJUBLJANA VECNA POT 111 BIOTECHNICAL FACULTY SI-1000 LJUBLJANA, SLOVENIA DEPARTMENT OF BIOLOGY contact email: Last change: 2007-02-01 1/1 PHOSPHATE BUFFERED SALINE (PBS) 10x PBS (sodium-potassium combo), 1 L: 2 g KCl g KH2PO4 80 g NaCl g Na2 HPO4 note: if Na2 HPO4 (7H2O), use , if Na2 HPO4 (12H2O), use Dissolve everything in approximately 800 ml of dH2O, then fill up to 1000 ml. Autoclave and store at room temperature. 10x PBS, pH 7 4 (sodium), 100 mL: 7,6 g NaCl (Mw g/mol, M = 1,3 mol/L) 0,99 g Na2 HPO4 (Mw g/mol, M = 0,07 mol/L) 0,41 g NaH2PO4 x H2O (Mw g/mol, M = 0,03 mol/L) Adjust pH with 1 M NaOH or 1 M HCl. Fill up to 100 ml. Autoclave and store at room temperature. Calculation of Mw: CHEMIX ( )

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