HiMedia Laboratories Pvt. Ltd. Certificate of …

1 HiMedia Laboratories Pvt. of analysis , quality and ConformityAppearance1 Colour of Powder Light yellow to pink Light pink2 Consistency homogeneous Complies3 Nature free flowing powder Complies-------------------------------- ---------------------------------------- ---------------------------------------- ---------------------------------------- -----------------------------Colour and Clarity of preparedmedium1 Colour of Medium Red coloured Complies2 Clarity of Medium clear solution in tubes Complies-------------------------------- ---------------------------------------- ---------------------------------------- ---------------------------------------- ----------------------------- Reaction Reaction of w/v aqueous solution at25 C. - - Response1 Cultural Response Cultural characteristics observed after anincubation at 35-37 C for 18- 48 hours. ---------------------------------------- ---------------------------------------- ---------------------------------------- ---------------------------------------- ----------------------Escherichia coli ATCC 259221 Inoculum (CFU) >=10 Complies2 Growth inhibited Complies-------------------------------- ---------------------------------------- ---------------------------------------- ---------------------------------------- -----------------------------Staphylococ cus aureus ATCC259231 Inoculum (CFU) 50-100 Complies2 Growth good-luxuriant Complies3 Colour of medium yellow Complies-------------------------------- ---------------------------------------- ---------------------------------------- ---------------------------------------- -----------------------------Staphylococ cus epidermidisATCC 122281 Inoculum (CFU)

2 50-100 Complies2 Growth fair-good Complies3 Colour of medium red Complies-------------------------------- ---------------------------------------- ---------------------------------------- ---------------------------------------- -----------------------------Material Code : M383AR No.: 040000304075 Material Name : Mannitol Salt BrothDate of Report : No : 0000115548 Exp. Date : Jun-2015 TESTRESULTSSPECIFICATIONSPAGE : 1/2 HiMedia Laboratories Pvt. of analysis , quality and ConformityInformation for BSE/TSE Risk The material was subjected to pH <= and/or a temperature in excess of 75 C for no lessthan 2 hours during the manufacturing process. The bovine raw material for this product was collected entirely fromIndian Origin animals in a licensed based establishment. The animals are inspected under a Govt. approved veterinarian'ssupervision and were apparently free from infectious and contagious diseases.

3 BSE (Bovine SpongiformEncephalopathy)/ TSE (Transmissible Spongiform Encephalopathy) and dioxine are not known to exist in India. Thismaterial does not contain, nor is derived from the specific risks material as defined in The Maharashtra AnimalPreservation Act Govt. of Maharashtra, India. STATUS OF THE MATERIAL : APPROVEDM aterial Code : M383AR No.: 040000304075 Material Name : Mannitol Salt BrothDate of Report : No : 0000115548 Exp. Date : Jun-2015 TESTRESULTSSPECIFICATIONSPAGE : 2/2 This is to certify that this lot passes and it confirms to the above mentioned tests and specifications . The informationgiven here is believed to be correct and accurate, however, both the information and products are offered without warrantyfor any particulars use, other than that specified in the current HiMedia manual or product document has been produced electronically and is vali