【輸血のための検査マニュアル】 Ver

1 ABO Rh(D) Rh Rh(D) ( ) D (weak D) ABO 2010 4 I 5% II 10 ABO Rh(D)

2 III 11 Rh(D) ( ) weak D ABO ABO IV ABO ABO Rh V VI 1 I 5% 1,200G 3, 000rpm 5 ( ) 1 L* 1 ( 50 L) 8 900 1,000G(3400rpm)

3 1mL* 3 5% * 12 75mm 15mm 1 2 3 1 50 L 1 50 L 1 2 ( ) 1mL 1mL 1mL 1mL 2 3 ( ) 900 1,000G(3,400rpm)15 100 125G(1, 000rpm )1 2/3 3 4 * 900 1,000G(3,400rpm) 15 100 125G(1, 000rpm ) 1 ( + 1+) 4+3+2+1+w+0mfH(PH) 12108520 1 ( )mf mixed field agglutination H hemolysis PH partial hemolysis Technical Manual 16th AABB 4+3+2+1+w+0mfH(PH) 12108520 1 ( )mf mixed field agglutination H hemolysis PH partial hemolysis Technical Manual 16th AABB ( )

4 3 II 1 ABO Rh(D) 1 1 1,200G(3, 000rpm ) 5 ( ) 2 7 1 3 4 3 5 5 A B D Rh (Rh cont) 6 ( ) 2 7 ( ) 8 5 Rh(D) 3 5 1 9 A1 B 1 10 11 900 1,000G(3,400rpm)15 100 125G(1, 000rpm )1 12 Rh ABO Rh(D) 1 1 1 1 ( ) 2 A1 1 3 5% ( ) Rhcont 900 1,000G(3,400rpm)15 100 125G(1, 000rpm )1 1 2 3 4 1 Rh 1 1 Rh(D) A 1 1 113311334422 1 1 1 1 ( ) 2 A1 1 3 5% ( ) Rhcont 900 1,000G(3,400rpm)15 100 125G(1, 000rpm )1 1 2 3 4 1 Rh 1 1 Rh(D) A 1 1 113311334422 4 2 ABO A B A1 B 0A 0 A A 0 B 0B B 00O O O AB 00AB AB 1 2 2 M P1 Lewis 3 (mf mixed field agglutination) 4 IV-1 Bombay(Oh) paraBombay(Ah Bh) ( H HI) O Rh(D) D Rh D Rh 0D 00D 0weak D D 00 Rh IV-3 D D 3 900 1,000G(3,400rpm) 15 100 125G(1, 000rpm )

5 1 D Rh 37 C15 60 3 4 Rhcont 3 5 IgG 1 900 1,000G(3,400rpm) 15 100 125G(1, 000rpm ) 1 D Rh 37 C15 60 3 4 Rhcont 3 5 IgG 1 5 ABO 37 C ABO ABO 3 3 2 ABO Rh(D) Rh(D) 37 C III-1 IV-2-3) Rh(D) O A B IgG A B ( ) IgG A B O O ABO III- ABO ABO ( 21 )

6 6 ( ) 3 5% ( ) EDTA ( ) 1 lL 7 8 900 1,000G (3,400rpm) 1 2 900 1,000G (3,400rpm)1 2 1mL 1mL( ) 3 5% 1mL 1mL 7 ( ) 3 5% 2 1 lL 1 7 8 900 1,000G (3,400rpm) 1 2 900 1,000G (3,400rpm)1 2 1mL( ) 3 5% 1mL 1mL 1mL 8 PEG polyethylene glycol LISS low-ionic-strength solution * - - 9 37 C (indirect antiglobulin test IAT) IAT IV-2 1,200G(3, 000rpm )5 ( ) ( ) 2 ( )

7 Dia 1 900 1,000G(3,400rpm)15 100 125G(1, 000rpm )1 10 2 37 C 10 15 11 3 4 12 2 13 900 1,000G(3,400rpm)15 100 125G(1, 000rpm )1 14 15 3 5 IgG 1 900 1,000G (3,400rpm)15 100 125G(1, 000rpm )1 IgG 1 2 3 37 C 5 10 4 - - 5 ABO 6 Bombay(Oh) paraBombay(Ah Bh) H HI H 10 PEG polyethylene glycol LISS low-ionic-strength solution * - - 11 III ( ) Rh D E c C e Kidd Jka Jkb Duffy Fya Fyb Diego Dia Dib Kell K Ss S s 37 C M Lea A1 P1 N Leb LebH Xga JMH Knops Cost Chido/Rodgers 37 C M Le - 37 C 60 Rh Kidd Duffy Diego S s Kell Rh( D)

8 37 C M Le - 37 C 60 Rh Kidd Duffy Diego S s Kell Rh( D) 12 Rh(D) ( ) weak D D D D D weak D D D D D D D D D 00weak D0 D ( ) weak D D ABO ABO O / AB ABO O / AB ABO ABO 1 37 C A1 B A O Bombay(Oh) 37 C H O Bombay(Oh) Bombay(Oh) A BA1 B O A1 B / 0 0 OA 0 00 AA000 0 A (+)AA0 0 OB0 0 0BB00 00 B (+)BB 00 BAB 000 ABAB 0 0 AAB 0 0 0 ABAB 0 OAB0 000 A (+)ABAB 0000 B (+)ABABB AB ( ) A A B 37 C A1 B 13 IV ABO ABO ABO ABO ABO 1 ( A1 H ) 1) 2) MDS 3) Hodgkin 2 37 C 37 C 37 C ( )

9 4 C 1 2-ME DTT 3 3 1) 2) 4 1) 5 1) ABO 2) ABO 6 A B ( IgM) 1) 2) 3) 7 1) M N Lea Leb A1 B P1 8 (1 ) 1) 2) 3) 4) (T ) A B Acquired B B ( ) 3 - A1 B 3 14 ABO ABO A B ( 3+) ABO ABO O A1 I