ZINC (Colorimetric Test EC REP with 5-Brom-PAPS)

1 IVDLOTREFoCoCEC REPSYMBOLS IN PRODUCT LABELLINGA uthorised RepresentativeFor in-vitro diagnostic useBatch Code/Lot numberCatalogue NumberConsult instructions for useTemperature LimitationUse by/Expiration DateCAUTION. Consult instructionsfor useManufactured byZINC (Colorimetric Testwith 5-Brom-PAPS) REF: 330 001 ( 2 x 25 ml) 50 testREF: 330 002 ( 4 x 25 ml) 100 testSpecimenSerum, Plasma or UrineSystem ParametersWavelength560 nmOptical path1 cmAssay typeColorimetric End pointSample : Reagent Ratio1 : 20 : Reagent volume1 ml Sample volume50 lTemperature25 oC or 37 oCZero adjustmentAgainst Reagent Blank (RBL)Linearity400 g/dl ( mmol/l)Procedure Blank Standard SampleReagnt (R) 1 ml 1 ml 1 mlStandard (St).

2 50 l ..Sample .. 50 lMix and incubate for 10 min at 25 oC or 5 min at 37 the absorbance of the sample As and the absorbance ofstandard Ast against reagent Concentration ( g/dl) = x 200 zinc Concentration ( mol/l) = x ControlNormal & abnormal control serum of known concentrations shouldbe analyzed with each ComparisonA comparison between Spectrum Diagnostics zinc reagent and acommercial reagent of the same methodology was performed on 20human correlation of was reaction is linear up to zinc concentration of 400 valuesSerum/PlasmaMale: - 127 g/dl ( - mol/l)Female: - 114 g/dl ( - mol/l)During pregnancy and menstruation the concentration of zinc canbe very lowChildren: - 110 g/dl ( - mol/l)New born.

3 - g/dl ( - mol/l)Urine 300 - 800 g/dlIntended UseSpectrum Diagnostics liquizyme zinc reagent is intended for the in-vitro quantitative, diagnostic determination of zinc in human serum,plasma or is an essential element in the nutrition of human beings, zincis required in the genetic make-up of every cell and is an absoluterequirement for all biologic is needed in all DNA and RNA syntheses and is required atevery step of the cell 2 grams of zinc is distributed throughout the body is a condition where insufficient zinc is available formetabolic needs. The defficiency may lead to Anorexia, Diarrhia andPneumonia or cognitive and motor function impairment in defficiency during pregnancy can negatively affect both themother and Method with PrincipleZinc forms with 2-(5-Brom-2-pyridylazo)-5-(N-propyl-N-su lfopropylamino)-phenol a red chelate increase of absorbance can be measured and is proportionalto the concentration of total zinc in the (St.)

4 200 g/dl ( mol/l)Reagent (R)5-Br-PAPS mmol/LBicarbonate buffer pH 200 mmol/LSodium Citrate 170 mmol/LDimethylglyoxime 4 mmol/LDetergent 1 %For further information, refer to the Aspartate aminotransferasereagent material safety data and WarningsDo not ingest or inhalate. In case of contact with eyes or skin; rinseimmediately with plenty of soap and water. In case of severe injuries;seek medical advice PreparationSpectrum zinc reagents are supplied Storage and StabilityAll reagents are stable until expiration date stated on label whenstored refrigerated at 2 - 8 Creative Approach to BioscienceAspecimenAstandardAspecimenAst andardWaste DisposalThis product is made to be used in professional consult local regulations for a correct waste :dispose of this material and its container at hazardous or special waste collection :use appropriate container to avoid environmental :avoid release in environment.

5 Refer to special instructions/safetydata Diagnostics does not interpret the results of a clinicallaboratory procedure; interpretation of the results is consideredthe responsibility of qualified medical personnel. All indicationsof clinical significance are supported by literature and Evaluation of a commercially availablekit for the colorimetric determination of Journal of Andrology, 1987, April 10 (2) , text book of clinical chemistry and molecular diagnosticsISBN 0-7216-0189-8 ORDERING INFORMATIONCATALOG NO. QUANTITY 330 001 2 x 25 ml 330 002 4 x 25 mlIFUFCC56 Rev.(1), 1/8/2009 MDSS GmbHSchiffgraben 4130175 Hannover, GermanyEC REPE gyptian Company for Biotechnology ( )Obour city industrial area.

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