SC400 Colorimeter - orbeco.com

1 Photometer-SystemSiO2 Instruction ManualDEGBFRITESB edienungsanleitungIstruzioni d'usoMode d'emploiInstruccionesSeite 3 17 Page 19 33 Page 35 49 Pagina 51 65P gina 67 81SC400 Colorimeter CODI nstruction Manual2SC400-00, COD 03/2009 RequirementsMethod: Oxygen Demand, ChemicalMethod Description: The sample is heated at 150o C for two hours in the presence of a potassium dichromate, which is a strong oxidizing agent. Organic compounds in the sample are oxidized, reducing the dichromate ion (Cr2O72 ) to the green chromic ion (Cr3+). In the Low Range (LR) Test (0-150 mg/L), the amount of remaining Cr6+ is measured; In the Mid Range (MR) (0-1,500 mg/L) or (HR) (0-15,000 mg/L), the amount of Cr3+ produced is measured. The results are measured as the amount of oxygen (O2) consumed per liter of water sample.

2 The LR and MR test is displayed in mg/L while the HR test shows g/L. The COD reagent contains both mercury and silver the mercury controls chloride interferences and the silver functions as a catalyst to speed the reaction. Required Instruments: SC400 Colorimeter for COD (P/N: SC400 -00) TR125 Thermoreactor (P/N: TR12500)Required Accessories: 16 mm Tube Adapter (P/N: L19802220)Required Reagents: Range: Reagent Description: Reagent P/N: 0-150 mg/L Low Range COD Tube Test, 25 Tests TT20720 OR Low Range COD Tube Test, 150 Tests TT20725 0-1500 mg/L Mid Range COD Tube Test, 25 Tests TT20721 OR Mid Range COD Tube Test, 150 Tests TT20726 0-15000 mg/L High Range COD Tube Test, 25 Tests TT20722 OR High Range COD Tube Test, 150 Tests TT20727 Alternate Reagents:For non-reporting purposes, our complete line of Mercury-Free COD reagents is available.

3 These reagents follow the same procedure and calibrations pre-programmed in the SC400 -00. These reagents are not acceptable for USEPA reporting : Reagent Description: Reagent P/N:0-150 mg/L Low Range COD Tube Test, Mercury-Free, 25 Tests TT20710 0-1500 mg/L Mid Range COD Tube Test, Mercury-Free, 25 Tests TT20711 Mid Range COD Tube Test, Mercury-Free,150 Tests TT20712 0-15000 mg/L High Range COD Tube Test, Mercury-Free, 25 Tests TT207163SC400-00, COD 03/2009 ContentsGeneral Notes ..4 Method notes ..4 Chemical method notes ..4 Battery change ..4 Special Functions ..5 Backlight ..5 Recall of stored data ..5 Measuring the absorbance ..5 Methods ..6 Tube Test Reagents.

4 6 Preparing Sample ..6 Measurement ..6 Guidelines for colorimetric measurement..7 Menu Options ..8 Menu selections ..8 Recall of stored data ..8 Setting date and time ..8 Calibration Mode ..9 User calibration ..9 Recommended calibration values ..9 Factory calibration reset..10 Technical Data ..11 Operating messages ..11 Error codes ..114SC400-00, COD 03/2009 Method notesPrior to measurement ensure that the sample is suitable for analysis (no major interferences, such as chloride-see below) and does not require any preparation pH adjustment, filtration are designed for use in chemical analysis only and should be kept out of the reach of children. Ensure proper disposal of reagent solutions. Material Safety Data Sheets: available on our website ( ) or upon method notes:Method:The organic material present in the sample is oxidizing by a standard amount of a potassium dichromate oxidized mixture.

5 After oxidation is complete, the excess of this reagent is measured :Samples can be measured if the chloride content does not exceed 1,000 mg/L (LR/MR) or 10,000 mg/L (HR).In rare cases, compounds contained in the water cannot be oxidized adequately. This results in low results compared with the reference methods of sampling, preparation of the sample itself and the time elapsed between taking the sample and analysis can all affect the obtained NotesCAUTIONThe accuracy of the instrument is only valid if the instrument is used in an environment with controlled electromagnetic disturbances according to DIN devices, wireless phones, must not be used near the instrument. Battery change5SC400-00, COD 03/2009 Special FunctionsAbSDisplay backlightPress the [ ! ] key to turn the display backlight on or off.

6 The backlight is turned off automatically during the of stored dataIf the instrument is turned on, press the [ ! ] key for more than 4 seconds to access the recall the absorbance This instrument ordinarily measures using two wavelengths. In the AbS mode, it is possible to measure absorbance at a specified : = 430 nm Abs2: = 605 nm!!6SC400-00, COD 03/2009 Measurement1. Insert the 16 mm adapter into the sample chamber by aligning the arrow on the adapter with the arrow on the instrument. Be sure that the adapter seats completely into the sample Turn on the unit using the [ON/OFF] display shows the following:3. Select the required test using the [MODE] key: LR (low range) MR (mid range) HR (high range) AbS1 (Absorbance 1) AbS2 (Absorbance 2) LR.

7 (Scroll)The display shows the following:4. Place the blank in the adapter (Note 1 4) making sure that the marks are aligned. Blanks are specially prepared for each individual test Press the [ZERO/TEST] "Range" symbol flashes for approximately 8 display shows the following: After zeroing, remove the vial from the LrOnOff Zero TestMethodModeCOD Tube Test ReagentsSelect the appropriate vial for the desired range:LR-Range, 25 Tests: 0 150 mg/L P/N TT20720MR-Range, 25 Tests: 0 1 ,500 mg/L P/N TT20721HR-Range, 25 Tests: 0 15 ,000 mg/L P/N TT20722 Preparing the Sample1. Open a reaction vial with a white cap and add the specified volume. (Ensure that appropriate personnel safety equipment is used.) LR-/MR: 2 ml water sample HR: ml water sample2.

8 Prepare a blank (Note 1) by using deionized water (TOC-free) instead of the sample (LR/MR: 2 ml, Hr: ml).3. Replace the cap tightly. Invert the vial gently several times to mix the contents (The vial will become hot during mixing!) and digest the vials for 120 minutes in the reactor at a temperature of 302 F (150 C). 4. Remove the vials from the reactor and allow them to cool down to 60 C or less. Mix the contents by inverting each vial several times while still warm. Then allow the vials to cool to ambient temperature before , COD 03/20091. Press the [ZERO/TEST] "Range" symbol flashes for approx. 3 result appears in the result is saved : in mg/L HR-range: in g/L (this unit is shown by an alternating display of the reading and g : L)Accuracy: % (full scale)Repeating the test: Press the [ZERO/TEST] key zero calibration: Press the [MODE] key until the desired range symbol appears in the display Zero Test Zero TestGuidelines for colorimetric measurementsRun samples and blanks from the same batch of vials.

9 1. The blank is stable when stored in the dark and can be used for further measurements with vials from the same 't place hot vials in the adapter. Allow the vials to cool to room temperature for 2. minimum 45 minutes. It is recommended to let the vials to cool solids in the vial lead to incorrect measurements. For this reason, it is important 3. to place the vials carefully in the adapter. The precipitant at the bottom of the sample should not be the outside of the vials with a towel to remove fingerprints and other Avoid spilling water in the sample chamber. If water should spill into the chamber, absorb 5. it with a soft cloth or tissue. Contamination of the optics (LED and photo-sensor) in the sample chamber will result in 6. incorrect measurements.

10 The windows in of the sample chamber must be checked at regular intervals and cleaned as necessary. Use a moistened cloth or cotton swab for cleaning temperature differences between the Colorimeter and ambient conditions can lead 7. to incorrect measurements, or build-up of condensation around the optics of the avoid errors caused by stray light do not use the instrument in bright Method8SC400-00, COD 03/2009 Menu OptionsOnOffMode!Mode!Mode!Time CalStore Date2341 ModeSETDATEYYYY (2 sec.)ModeMenu selections1. Press the [MODE] key and hold. 2. Turn on the unit using the [ON/OFF] key. Allow the 3 decimal points to be displayed before releasing the [MODE] [!] key allows for selection of the following menu points: 1 recall stored data 2 3 setting the date and time 4 user calibrationThe selected menu is indicated by an arrow in the the selection with the [MODE] Recall of stored dataThe Colorimeter shows the last 16 data sets in the following format (automatically proceeds every 3 seconds until result is displayed):Number n xx (xx: ) Year YYYY ( 2008) Date (monthmonth:dayday) Time hh:mm (hourhour:minuteminute) Test Method Result x,xxThe [ZERO/TEST] key repeats the current data [MODE] key scrolls through all stored data the menu by pressing [!]