Acetone Precipitation Of Proteins And
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Acetone precipitation of proteins - Thermo Fisher Scientific
tools.thermofisher.com7. Allow the acetone to evaporate from the uncapped tube at room temperature for 30 minutes. Do not over-dry pellet, or it may not dissolve properly. 8. Add buffer appropriate for the downstream process and vortex thoroughly to dissolve protein pellet. TR0049.1 Acetone precipitation of proteins
Acetone precipitation of proteins - Thermo Fisher Scientific
tools.thermofisher.com7. Allow the acetone to evaporate from the uncapped tube at room temperature for 30 minutes. Do not over-dry pellet, or it may not dissolve properly. 8. Add buffer appropriate for the downstream process and vortex thoroughly to dissolve protein pellet. TR0049.1 Acetone precipitation of proteins
Ethanol Precipitation of Protein: Protocol and % Recovery
kendricklabs.comEthanol Precipitation of Protein: Protocol and % Recovery Introduction Sodium Dodecyl Sulfate (SDS) is an ionic detergent used in biological buffers to dissolve and denature proteins. Unfortunately, it interferes with many procedures and tests such as immuno-precipitation and mass spectrometry.
A Rapid and Sensitive Method for the Quantitation of ...
hoffman.cm.utexas.eduprocedure, too, has its drawbacks since the precipitation of the protein by trichloroacetic acid followed by filtration on Millipore membranes is required. The protein assay herein described eliminates most of the problems involved in the procedures described above, and is easily utilized for 248