Agar Agar
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Preparation of culture media, agar plates, antibiotics and ...
2015.igem.orgWith 200 ml LB-agar you can make 8 plates (1 l for ~40 plates). • For 200 ml LB-agar, the correct amounts are: 1 g yeast extract 2 g NaCl 2 g peptone 3 g bacto-agar • Collect them in in a bottle and add 200 ml of H. 2. O. • Autoclave the LB-agar at 121 °C for 20 minutes (sterilisation). 2.2 . Pouring the plates
Indole Test Protocol
asm.orgAgar 2.0 g Bromcresol purple 0.02 g Bring to 1 liter with distilled water. Heat mixture to boiling to dissolve agar. Cool to 50°C. Dispense in 4.0 to 5.0 ml aliquots in 16-mm test tubes. Cap tubes and autoclave at 121°C under 15 psi pressure for 15 minutes. After autoclaving, allow tubes to cool in an upright position to form the agar deep.
Lactobacilli MRS Agar • Lactobacilli MRS Broth
legacy.bd.comLactobacilli MRS Agar and Lactobacilli MRS Broth are based on the formulations of deMan, Rogosa and Sharpe.1 These media were shown by the authors to support luxuriant growth of all lactobacilli from oral, fecal, dairy and other sources. Principles of the Procedure Lactobacilli MRS Agar and Lactobacilli MRS Broth contain peptone and dextrose.
Isolation &Characterization of Bacteria
www.gbiosciences.comthe LB agar plate. Gentle press the swab on to the LB agar plate neat one edge. Add 10 l sterile water or PBS to the area where the swab was placed and then streak the plate as described in step 5. 3. Leave the tube at room temperature for 1 minute. 4. Write your group name on the bottom of the agar plate.
MacConkey Agar - himedialabs.com
www.himedialabs.comMacConkey Agar is the earliest selective and differential medium for cultivation of coliform organisms (1, 2). Subsequently MacConkey Agar and Broth have been recommended for use in microbiological examination of foodstuffs (3) and for direct plating / inoculation of water samples for coliform counts (4).
THE IDENTIFICATION OF TWO UNKNOWN BACTERIA
openlab.citytech.cuny.edu• Blood Agar (BA) to determine the hemolytic properties of both unknown bacteria • MacConkey Agar (MAC) that will select for the gram-negative bacterium and also determine whether or not it is a lactose fermenter. Colonies produced on this plate will also be used for biochemical testing to determine its identification.
BD TCBS Agar
legacy.bd.comBD TCBS Agar, preparado según la fórmula de Kobayashi et al, es una modificación del medio selectivo de Nakanishi4,5. Todas las especies de Vibrio patógenas para los seres humanos, excepto V. hollisae, crecen en este medio. Este medio se recomienda para el aislamiento de las
Kirby-Bauer Disk Diffusion Susceptibility Test Protocol
asm.orgagar for 100-mm plates and 60 ml of liquid agar for 150-mm plates, but in any case to a measured depth of 4 mm). Plates that are too shallow will produce false susceptible results as the antimicrobial compound will diffuse further than it should, creating larger zones of .