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A Beginner’s Guide to Bioprocess Modes – Batch, Fed- Batch, …
www.eppendorf.comand ampicillin were also aseptically added to reach a final concentration of 90 g/L and 0.1 g/L, respectively (Table A1). For fed-batch fermentation, we used chemically defined medium as well, as described in Table A1. We prepared 1.5 L feeding medium containing MgSO 4, thiamine, trace elements, and 614 g/L glucose.
ANTIBIOTIC SENSITIVITY TESTING I. OBJECTIVES II.
www.sas.upenn.eduused are: gentamicin, tetracycline, penicillin G, chloramphenicol, ampicillin and erythromycin. 7. Repeat the alcohol-flame sterilization of the forceps and tap each disc gently onto the plate. 8. Replace the lid, and invert the plate. Complete the label at the bottom of plates and incubate at 37°C for 2 days. 9.