Electrophoresis Buffers
Found 8 free book(s)
Protocol: Protein electrophoresis and western blot recipes
assets.thermofisher.comSample preparation buffers Electrophoresis running buffers Ready-to-use alternative: Invitrogen ™ NuPAGE LDS Sample Buffer (4X) (Cat. No. NP0007) Ready-to-use alternative: Invitrogen ™ Novex Tris-Glycine SDS Running Buffer (10X) (Cat. No. LC2675)
Recipes for stock solutions and general use buffers
people.virginia.eduBuffers for agarose gel electrophoresis 50x TAE: For 1 L • Dissolve 242.2 g Tris base in around 600 mL of ddH 2O • Slowly add 57.1 mL glacial acetic acid • Add 100 mL 0.5 M EDTA, pH 8 • Bring up the volume to 1 L with ddH 2O 6x DNA loading buffer: For 100 mL • Weigh 60 g …
A Guide to Polyacrylamide Gel Electrophoresis and Detection
www.bio-rad.comBuffers and Salts 21 Common Solutions for Protein Solubilization 21 Removal of Interfering Substances 21 Immunoprecipitation 22 Sample Quantitation (Protein Assays) B22 ... electrophoresis include the strength of the electric field, the …
Capillary Electrophoresis in Quality Control: Part I ...
sciex.com31.2 cm; both anode and cathode buffers were SCIEX CE-SDS gel solution. Samples were injected at a constant electric field of 160 V/cm for 20 s and electrophoresed at 480 V/cm (32.5 μA) and 40°C. (Reprinted from Reference 3, with permission). detection. The second advantage is that while the capillary is
Agilent 2200 TapeStation System User Manual
www.agilent.comoptimised buffers for the effective separation of nucleic acid fragments or proteins. Electrodes: The integrated electrodes apply a current across the ScreenTape device and eliminate the need for any additional electrophoresis equipment. Gel: The gel contained within the ScreenTape device has been developed
Buffer Formulations - Bio-Rad
www.bio-rad.comThis is an excerpt from Bio-Rad’s comprehensive Electrophoresis Guide (Bulletin 6640). Running Buffers Buffer Components 10× SDS-PAGE (1 L) (cata log #161-0732) 250 mM Tris, 1.92 M glycine, 1% SDS, pH 8.3 Tris base 30.30 g Glycine 144.10 g SDS 10.00 g diH 2O to 1 L Do not adjust the pH (~pH 8.3). ...
General western blot protocol - Abcam
docs.abcam.comLysis buffers These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year. NP-40 buffer ‒ 150 mM NaCl ‒ 1.0% NP-40 (possible to substitute with 0.1% Triton X-100) ‒ 50 mM Tris-HCl, pH 8.0 ‒ Protease inhibitors RIPA buffer (radioimmunoprecipitation assay buffer) ‒ …
PCR clean-up Gel extraction - Takara Bio
www.takarabio.comborate electrophoresis systems. • Concentrated elution in down to 15 μL Elution Buffer NE (details in section 2.6). • ®Several support protocols extend the application range of NucleoSpin Gel and PCR Clean-up to - Clean-up of DNA from reaction mixtures containing SDS (section 5.5) - Clean-up of single stranded DNA (section 5.6)