Ligation Dna Ligation
Found 3 free book(s)One Shot TOP10 Chemically Competent E. coli
assets.thermofisher.com1. Centrifuge the ligation reactions briefly and place on ice. 2. Thaw, on ice, one 50 μL vial of One Shot™ cells for each transformation. 3. Pipet 1–5 μL of each ligation reaction into the vial of competent cells and mix by tapping gently. Do not mix by pipetting up and down. Store remaining ligation reactions at –20°C. 4.
pET System Manual - Fred Hutch
research.fredhutch.orgLigation-independent cloning was developed for the directional cloning of PCR products without restriction enzyme digestion or ligation reactions (Aslanidis and de Jong, 1990; Haun et al., 1992). LIC vectors are created by treating a linearized backbone with T4 DNA polymerase in the presence of only one dNTP. The 3'→5' exonuclease activity of ...
Chapter 7: Detection of Cholera Toxin
www.cdc.govAfter exteriorization and ligation of the rabbit’s small intestine, a cellfree supernatant is inject- ed into each ileal loop and the abdomen is closed for 18 hours. The rabbit then euthanized, the intestine removed, and the loops ... The DNA amplicon is defined by the location of two short, specific DNA oligonucleotides