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Ligation Dna Ligation

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One Shot TOP10 Chemically Competent E. coli

One Shot TOP10 Chemically Competent E. coli

assets.thermofisher.com

1. Centrifuge the ligation reactions briefly and place on ice. 2. Thaw, on ice, one 50 μL vial of One Shot™ cells for each transformation. 3. Pipet 1–5 μL of each ligation reaction into the vial of competent cells and mix by tapping gently. Do not mix by pipetting up and down. Store remaining ligation reactions at –20°C. 4.

  Ligation

pET System Manual - Fred Hutch

pET System Manual - Fred Hutch

research.fredhutch.org

Ligation-independent cloning was developed for the directional cloning of PCR products without restriction enzyme digestion or ligation reactions (Aslanidis and de Jong, 1990; Haun et al., 1992). LIC vectors are created by treating a linearized backbone with T4 DNA polymerase in the presence of only one dNTP. The 3'→5' exonuclease activity of ...

  Ligation

Chapter 7: Detection of Cholera Toxin

Chapter 7: Detection of Cholera Toxin

www.cdc.gov

After exteriorization and ligation of the rabbit’s small intestine, a cellfree supernatant is inject- ed into each ileal loop and the abdomen is closed for 18 hours. The rabbit then euthanized, the intestine removed, and the loops ... The DNA amplicon is defined by the location of two short, specific DNA oligonucleotides

  Ligation

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