Protocols And Tips In Protein Purification
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IMMUNOPRECIPITATION (IP) PROTOCOL
www.abcam.comImmunoprecipitation is a method that enables the purification of a protein. An antibody for the protein of interest is incubated with a cell extract so that the antibody will bind the protein in solution. The antibody/antigen complex will then be pulled out of the sample using protein A/G-coupled agarose beads.
pSUPER : Manual A Vector System for Expression of Short ...
www.oligoengine.comOur experience demonstrates that gel purification of the oligos is not necessary for efficient ligation. Dissolve the oligos in sterile, nuclease-free H2O to a concentration of 3 mg/ml. If you need assistance to determine how much H2O (or buffer, etc.) to add to your product on hand, see the Lab Tips section in this manual following the procedure.
Sucrose density gradient centrifugation for efficient ...
nvlpubs.nist.govexposure rinsing strategy that, unlike conventional water rinsing protocols, ensured greater efficiency in regard to the removal of excess, non-ingested ENPs. Density gradient centrifugation is widely considered one of the most convenient and simple methods for NP separation for the purposes of purification, size selection, E shape and and