Sample Protocols And Experiments Guide

Real-Time PCR Applications Guide - Bio-Rad

This guide provides an introduction to many of the technical aspects of real-time PCR. It includes guidelines for designing the best real-time PCR assay for your experiments and explains how real-time PCR data are used in various applications. In Sections 5–7, we present sample protocols and data that demonstrate the use

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qPCR Quantification Protocol Guide - Boston University

qPCR Quantification Protocol Guide Introduction This document describes a qPCR method for quantifying libraries generated using the Illumina® sample preparation protocols. qPCR is a method of quantifying DNA based on PCR. qPCR tracks target concentration as a function of PCR cycle number in order to derive a quantitative estimate of the

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RNA extraction from tissue - PROTOCOL using Bioruptor ...

Troubleshooting guide Problem Possible cause Suggested solution Low yields Expected yield of RNA per mg of tissue: Liver 4-10 μg Skeletal muscle 0.3-1 μg Brain 2-5 μg HeLa cells 6-15 μg per 1X10^6 cells RNA is not solubilized completely Do not allow pellet to dry completely. Do not lyophilize or vacuum dry sample.

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Thin Layer Chromatography (TLC) - Delhi University

Figure 1 Schematic representation of ascending development chamber for conventional TLC (side-on view). Figure 2 (A) Mixture of A and B adsorbed on the stationary phase and free in mobile phase and (B) schematic representations of the principle of separation. STRATEGIC QUESTIONS Before performing a TLC experiment and the subsequent analysis, the following …