Study Aseptic Transfer And Material Handling
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PURE CULTURE TECHNIQUES I. OBJECTIVES
www.sas.upenn.edu• To demonstrate good aseptic technique in culture transfer or inoculation and in handling sterile materials. • To demonstrate skill in isolation of organisms from a mixed culture using selective and differential media. • To isolate microorganisms from a wide variety of sources and describe their colonial morphology. II. INTRODUCTION
GOOD LABORATORY PRACTICES
pharmexcil.comthere should be two aseptic zones having class 1000 ... one for inoculation and cultures transfer and another for sterility testing. For units having only non-sterile products, one aseptic zone shall be there. Laboratory Infrastructure ... should be made compulsory in the handling of corrosive chemicals. There should be adequate fire fighting
PTCB Study Guide - Fitzgerald Pharmacy Technician Class
fitzgeraldpharmacytech.weebly.comAseptic Technique’s dos and don’ts How to clean How to correctly operate Differences between Horizontal and Vertical Flowhoods Where the filter is located Direction of airflow Which one to use with hazardous materials Syringes/Needles – Names and …
ISOLATION OF PURE CULTURES - Western Washington …
fire.biol.wwu.eduthe aseptic technique. Asepsis can be defined as the absence of infectious microorganisms. However, the term is usually applied to any technique designed to keep unwanted microorganisms from contaminating sterile materials. FIRST PERIOD Material: 1. Seven 9-ml dilution tubes of sterile saline 2. Seven nutrient agar plates 3. 1.0 ml and 0.1 ml ...
Food Processing Plant Design & Layout - AgriMoon
agrimoon.comsubsequent moisture and oxygen transfer. Segregation often causes problems in the packaging of powdered foods. Aseptic packaging is starting to be widely used. Food processing techniques and formulations are sometimes constrained by standards of identity and good manufacturing practice regulations and codes.
Bacterial Culture & Growth - G-Biosciences
www.gbiosciences.comTransfer 1ml LB broth to a 1.5ml tube to be used as a blank later. 2. Inoculate the remaining LB broth with 5ml overnight culture that will be supplied by your teacher. Gently swirl the flask in a circular motion to thoroughly mix. 3. Transfer 1ml of your culture to a cuvette.Using a spectrophotometer at 600r