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Lab 2 Spectrophotometric Measurement of Glucose

Lab 2 Spectrophotometric Measurement of Glucose

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Reagent. The amount of colored substance is directly proportional to the amount of glucose present. You will create a standard curve that translates the absorbance of the colored substance into concentration of glucose and will use it to determine the amount of glucose in Kool-Aid. 1.

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Lab 2 Spectrophotometric Measurement of Glucose

Lab 2 Spectrophotometric Measurement of Glucose

www.employees.csbsju.edu

into concentration of glucose and will use it to determine the amount of glucose in Kool-Aid. 1. First you will make several dilutions of a glucose standard solution that has an initial concentration of 500 mg/dl of glucose. See Fig. 2-3. a. Label three large mixing tubes A, B, C. b. Into each of the large mixing tubes, pipette 5 ml of ...

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Determination of Glucose by Titration with Fehling’s ...

Determination of Glucose by Titration with Fehling’s ...

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Fehling’s test for reducing sugars has been used since the 1800’s to determine the amount of glucose and other reducing sugars (lactose in milk, for example). It has had many applications including use in agriculture (glucose determination in corn for use in corn syrup) and in medicine (glucose determination in urine for diabetes tests).

  Determine, Glucose, Amounts, To determine the amount of glucose, Of glucose

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