Search results with tag "Bovine serum albumin"
PierceA Protein Assay Kit BC - Thermo Fisher Scientific
assets.thermofisher.com23209 Albumin Standard Ampules, 2mg/mL, 10 × 1mL ampules, containing bovine serum albumin (BSA) 23208 Pre-Diluted Protein Assay Standards: Bovine Serum Albumin (BSA) Set, 7 × 3.5mL 23212 Bovine Gamma Globulin Standard, 2mg/mL, 10 × 1mL ampules 23213 Pre-Diluted Protein Assay Standards, (BGG) Set, 7 × 3.5mL aliquots
Research Article the Bovine Serum Albumin Protein ...
globalresearchonline.netBovine Serum Albumin Assay (BSA) The anti-inflammatory activities of the crude and fractionated plant extracts were determined using a modified version of the BSA assay reported by Williams et al.11 BSA solution (0.4%, w/v) was prepared in Tris Buffered Saline (one tablet is dissolved in 15 mL of
INSTRUCTIONS Micro BCA Protein Assay Kit
assets.thermofisher.com23235 Micro BCA Protein Assay Kit, sufficient reagents for 480 tube assays or 3200 microplate assays . Kit Contents: Micro BCA Reagent A (MA ... Bovine Serum Albumin Standard Ampules, 2mg/mL, 10 × 1mL ampules containing bovine serum albumin (BSA) at 2.0mg/mL in a solution of 0.9% saline and 0.05% sodium azide . Storage: Upon receipt store ...
Protein Analysis-Determination of Protein Concentration
public.callutheran.eduSep 06, 2013 · Bovine Serum Albumin (BSA): 2.0 mg/ml (stock) Ovalbumin (stock, unknown concentration) Lysozyme (stock, unknown concentration) Methods For each of the three sets of assays, we want to prepare a BSA standard curve, and dilutions of Ovalbumin and Lysozyme. (9 samples in all for each assay). We will calculate the protein
TR0006.4 Introduction - Thermo Fisher Scientific
tools.thermofisher.com23210 Albumin Standard Ampules, 2mg/mL, 50mL, containing bovine serum albumin (BSA) at a concentration of 2.0mg/mL in 0.9% saline and 0.05% sodium azide . 23212 Bovine Gamma Globulin Standard Ampules, 2mg/mL, 10 × 1mL . 23225 BCA Protein Assay Kit, sufficient reagents for 500 test tube or 5000 microplate assays . 23235 Micro BCA Protein Assay Kit
1-800-4BIORAD Protein Assay Bio-Rad
www.bio-rad.comassays using the standard assay procedure, or 2,250 assays using the microassay procedure. The Dye Reagent Concentrate can be purchased in a kit with one of two standards: Bovine gamma globulin (Kit I, catalog number 500-0001) or bovine serum albumin (Kit II, catalog number 500-0002). The Bio-Rad Protein Assay is for research use only.
Quick Start Bradford Protein Assay - Bio-Rad Laboratories
www.bio-rad.comas bovine serum albumin (BSA) and bovine 4 4110065A.qxp 9/25/2007 2:39 PM Page 10. ... 1.2 Selecting a Protein Standard In any protein assay, the ideal protein to use as a standard is a purified preparation of the protein being assayed. In the absence of such an absolute reference protein, another protein must be selected as a relative standard ...
Lowry Protein Assay - University of Florida
animal.ifas.ufl.eduStandard: Bovine serum albumin (BSA), lysozyme or other protein. If the standard is weighed out before use, make sure that the protein preparation used to prepare the standard is salt-free to avoid inaccurate results. The standard should be dissolved at a concentration of 1 mg/ml in a buffer similar to the solution
A COMPARISON OF BIURET, LOWRY AND BRADFORD …
www.mnet.mendelu.cz• Solution of bovine serum albumin. • Coomassie Brilliant Blue G-250: 10 mg of Coomassie G-250 was dissolved in 5 ml of ethanol, after concentrated phosphoric acid (10 ml) and 85 ml …
Experiment 3: Activity Determination
www.sites.psu.eduunknown mixture is needed. A “Mystery Mix” containing glucose, bovine serum albumin (BSA), and the enzyme acid phosphatase was used to determine the specific activity of the enzyme in the unknown mixture. First, the Bradford Method was used to determine the amount of protein present in the mixture using a colored dye called Bradford Reagent.
A theoretical and practical guide for spectrophotometric ...
assets.thermofisher.comvalues are currently available for bovine serum albumin (BSA). With a quick online search, several values reported in the literature can be found (Table 1). Overall, mass extinction coefficients are easier numbers to handle, so they are frequently used. ε 0.1% is especially convenient because it provides absorbance values for a
A Rapid and Sensitive Method for the Quantitation of ...
hoffman.cm.utexas.eduWhen bovine serum albumin is used as the standard in the micro assay system the degree of nonlinearity is similar to that found in the standard assay. There is a loss in protein-dye complex response as compared with the standard assay, i.e., 5 pg protein/ml gives an absorbance change of 0.1 vs 0.27 in the standard as- ...
Phosphate Buffered Saline pH 7 - HiMedia Labs
himedialabs.comPhosphate Buffered Saline pH 7.4 With 1% Bovine Serum Albumin Product Code: TS1120 Please refer disclaimer overleaf Product Description : All media used in tissue culture have a basis of a synthetic