Transcription of 黄色ブドウ球菌試験法 最終案 091117 - nihs.go.jp
1 1 091117 2 NIHSJ-03-ST4 ISO6888-1 (coagulase-positive staphylococci (Staphylococcus aureus and other species)) BPW BPW 10 2 1g ISO6888-1 Baird-Parker Baird-Parker 37 C 25 50 C 37 C H m 90 mm 3 1 2 10 ml 4-1 X 9
2 BPW 9X ml 10 10 10 100 1,000 4-2 4-2-1 Baird-Parker ml Baird-Parker 15 37 1 C 48 24 24 1 mm 48 mm 24 300 150 1 15 4-2-2 Baird-Parker Baird-Parker ml 48 4 1 2 mm 4-3 4-3-1
3 37 1 C 22 4-3-2 4-3-2-1 4-3-2-2 ml BHI 37 1 C 22 BHI ml 37 1 C 24 1 4 24 Baird-Parker 5 4-4 4-4-1 ISO 7218:2007(E) NIHSJ-18 5-1 Buffered pepton water (BPW)
4 BPW 5-2 Baird-Parker 5-2-1 5-2-2 Potassium tellurite solution 5-2-3 Egg yolk emulsion 30 5-2-4 100 ml ml 30 ml 121 C15 50 C 4 mm 90 mm 20 ml C 24 25 50 C 5-3 5-3-1 6 1,000 ml 900 ml 5-3-2 121 C15 5-2-3 100 ml 5-4 TSB NaCl 5-5 TSA 5-6 BHI 5-7 5-8 7 1 X g 9 9X ml 10 10 100 1.
5 000 ml Baird-Parker * 37 1 C 48 2 5 TSA 37 1 C 22 1 g * 8 1 2 5 TSA 37 1 C 22 ml BHI broth 37 1 C 22 ml 37 1 C 4 24 9 1. Buffered pepton water (BPW) [M192] 10 g NaCl 5 g Na2 HPO4 g KH2PO4 g 1,000 ml pH 121 C 15 BPW 2.
6 Baird-Parker 2-1 Oxoid CM0275 2-2 1 Potassium tellurite solution K2Te O3 g 100 ml m 4 C 1 Oxoid SR030 Tryptone g g g g L- g g 12 22 g 1,000 ml pH 102-3 20 Egg yolk emulsion 70 30 4 4 C 3 Oxoid SR047 30 2-4 Sulfamezathine (sulfamethazine, sulfadimidine) Proteus g NaOH 10 ml 90 ml m 4 C 1 2-5 100 ml ml ml ( ml)
7 121 C15 50 C 4 mm 90 mm 20 ml 11 C 25 50 C 3. Baird-Parker 3-1 1 g 10 g NaCl 75 g 10 g 15 g 12 ml 850 ml H 3-2 121 C15 2-3 150 ml 30 900 ml 100 ml 4.
8 TSB NaCl 5. EDTA
