第2章 RNAの世界 富田 耕造 - science-academy.jp

1 2 RNA RNA DNA RNA 1960 F. H. C. Crick DNA DNA DNA 1 DNA mRNA RNA DNA DNA RNA DNA RNA

2 DNA 1980 RNA RNA RNA RNA RNA RNA Ribonucleic Acid Enzyme Ribozyme 2 RNA- - RNA RNA RNA DNA RNA DNA RNA DNA RNA RNA RNA RNA RNA RNA RNA DNA DNA RNA DNA RNA RNA intron

3 Exon Splicing RNA DNA DNA DNA RNA RNA RNA RNA RNA editing : RNA RNA RNA DNA DNA RNA DNA RNA DNA DNA RNA 3 RNA RNA DNA RNA 1990 RNA 20 22 RNA

4 RNA RNA RNA interference: RNAi RNA RNA rRNA RNA ribosomal RNA tRNA RNA transfer RNA RNA 20 30 RNA RNA 1950 J. Watson DNA 20 1980 RNA 21 RNA RNA RNA DNA RNA 1950 DNA DNA DNA deoxyribonucleic acid deoxyribonucleoside

5 Ribonucleoside RNA (ribonucleic acid) 4 O- 4 DNA RNA RNA DNA 4 2 2 DNA 3 CH2 5 5 RNA mRNA RNA messenger RNA mRNA tRNA RNA transfer RNA rRNA RNA: ribosomal RNA RNA RNA rRNA 80% tRNA % mRNA % RNA RNA 5 snRNA (small nuclear RNA) rRNA snoRNA small nucleolar RNA tRNA RNaseP RNA (ribonuclease P RNA) tmRNA transfer-messenger RNA RNA ncRNA non-coding RNA RNA miRNA (micro RNA)

6 RNA RNA 5 RNA RNA RNA DNA RNA RNA DNA DNA RNA RNA RNA mRNA RNA mRNA DNA RNA 1980 T. Cech S.

7 Altman RNA rRNA (GTP) 3 5 3 5 3 3 ( 6) 6 rRNA P P9 RNA tRNA 5 P P RNA M1 RNA P M1 RNA tRNA 7 RNA

8 RNA Ribozyme RNA W. Gilbert RNA RNA RNA RNA RNA RNA RNA RNA 8 RNA RNA RNA RNA RNA RNA mRNA RNA 5 RNA tRNA

9 RNA A G U C RNA 20 7 RNaseP RNA RNase P RNA tRNA RNase P RNA tRNA 8 RNA RNA tRNA RNA RNA RNA W. Gilbert RNA RNA DNA RNA tRNA CCA tRNA tRNA tRNA ( 9)

10 TRNA RNA mRNA mRNA 9 tRNA tRNA tRNA 2 tRNA RNA RNA tRNA RNA M. Yarus RNA RNA SELEX Systematic Evolution of Ligands by Exponential Enrichment RNA RNA RNA RNA RNA DNA DNA DNA PCR