9 - Dewars and cryo accessories

1 This series of liquid nitrogen Dewars is based on a proven designand construction and provides a range of capacities to suit individuallaboratory storage and liquid nitrogen handling Dewars combine the qualities of strength, high thermalefficiency and ease of - Dewars and cryo accessoriesTechnical specifications are shown in the table below:Aluminium Dewars 2, 5 and 10 litreThese Dewars are ideally suited to applications where smaller quantities of liquid nitrogenare required. They are lightweight, portable and supplied with plug cap and carryinghandle. Low static evaporation rates are achieved through high vacuum and multilayer Dewar. 2 litreB7458 Aluminium Dewar. 5 litreB7465 Aluminium Dewar. 10 litreHeavy duty Dewar 25 litreThis heavy duty, stainless steel Dewar is ideally suited to the more rigorous environment of industrialapplications.

2 A roller base is available for ease of duty stainless steel Dewar. 25 litreB797 Roller base for B796B798 TTrolley/pouring frame for B796 Aluminium Dewars 20 and 25 litreThese Dewars are made of aluminium. The 20 litre Dewar has the option of a roller base for ease oftransportation. The 25 litre Dewar has support trunnions to enable use with a trolley/pouring nitrogen Dewar. 20 litreB777 Roller base for B776B778 ALiquid nitrogen Dewar. 25 litreB798 TTrolley/pouring frame for B778 ALiquid nitrogen storage Dewars215 Cat. Capacity, litres 2 510202525 Evaporation rate, Static holding time, days 25 28 50 111 100 41 Weight empty, kg Weight full, kg Neck diameter, mm 32 51 51 51 51 Overall height, mm 385 495 560 570 585 600 Overall diameter, mm 185 216 305 407 395 4009_Dewars_Cryo_0510:Layout 1 17/6/10 16:47 Page 215 Tel:+44 (0)1279 813519 Fax:+44 (0)1279 815106 and cryo accessoriesPolypropylene DewarEnamelled steel DewarsThis low cost, one litre capacity, wide neck Dewar, with a tough blue polypropylene shock-absorbing container,is easily dismantled to replace the insert.

3 It includes a coated carrying handle and an insulated, vented, push-fit 1 litre, in polypropylene case with lidB7449 Replacement glass insertThese are very useful for handling the small quantities ofliquid nitrogen needed for freezing samples or for topping up anti-contamination Dewars . They all have quality borosilicate glass inserts protected bypolypropylene or metal cases. All glass inserts are range of rust and corrosion resistant Dewars with cushioned bases is available with wide or narrow necks, either with or without a folddown handle and insulated handle and lidCapacityIDODI nsideOverall(litres)(mm)(mm)depthheight( mm)(mm)B7442 Enamelled 85116185228B7441 Enamelled glass insert. 1 litreB7448 Replacement glass insert. 2 litreB7587 Replacement glass insert. litreB7588 Replacement glass insert.

4 10 litreSmall liquid nitrogen DewarsWithout handle and lidCapacityIDODI nsideOverall(litres)(mm)(mm)depthheight( mm)(mm)B7590 Enamelled glass inserts are available for all models; add suffix R to catalogue :Layout 1 17/6/10 16:47 Page 216217 Dewars and cryo accessories * These models have volume level lines on the inside of the Dewar to indicate the volume of are available for these models, add suffix L to catalogue steel DewarsThese are high quality Dewars with a carrying handle and stainless steel outer case. Toggle clamps secure the lid to the body for (litres)(mm)(mm)depthheight(mm)(mm)B7446 S/steel many applications it is advantageous to have well-insulated shatter proof Dewars with a wide neck to allow easy access for introducingarticles to be cooled. A carrying handle is provided for safety in transportation on all models except (litres)(mm)(mm)depthheight(mm)(mm)B7451 S/steel *85107206232B7453S/steel *100122157177B7454S/steel *100122285313B7455S/steel glass insert.

5 1 litreB7448 Replacement glass insert. 2 litreB7587 Replacement glass insert. litreB7584 Replacement glass insert. litreLiquid nitrogen carriersThese storage vessels are shatterproof and ideal for the transport and short term storage of liquidnitrogen. They are constructed from double walled high density polyethylene with urethane foaminsulation and will withstand temperatures down to -196 C, with a storage time of 1 - 2 days. Fittedwith a pouring spout, insulated cover and carrying nitrogen carrier. 1 litreB7476 Liquid nitrogen carrier. 2 litreB7477 Liquid nitrogen carrier. 4 litre9_Dewars_Cryo_0510:Layout 1 17/6/10 16:47 Page 217 Tel:+44 (0)1279 813519 Fax:+44 (0)1279 815106 and cryo accessoriesFace shieldA protective anti-splash face shield is recommended where the possibility of excessive boiling, sprayingor splashing of low temperature liquids is a shieldCryo aprons Essential for protection when handling low temperature from the same material as the cryogloves (C863/4/5/9), they have fully adjustable apron, small, 910 mm longC8051 cryo apron, medium, 1060 mm longC8052 cryo apron, large, 1210 mm longC8053 cryo apron, extra large, 1280 mm longCryoglovesThese gloves have been designed for use with ultra-low temperature freezers, cryostats, cold rooms andfreezers for handling dry ice and for retrieving materials from liquid nitrogen.

6 They provide protection to-135 C. The gloves retain flexibility, even after long periods in a cold atmosphere, and retain warmthwhile damp. They are machine washable and waterproof, but must not be immersed in liquid styles are available: wrist length 305 mm (12"), mid-arm length 355 mm (14"), elbow length 483 mm (19") and shoulder length 1020 mm (40").C863 Cryogloves, wrist length, medium*. PairC864 Cryogloves, mid-arm length, medium*. PairC865 Cryogloves, elbow length, medium*. PairC869 Cryogloves, shoulder length, medium*. PairThese cream hide cryogenic gloves are fully lined with 3 MTMT hinsulateTM, have knitted wrists and hidevein patches and are front seams. Ideal for handling liquid transfer cryogenic gloves. PairWith blue-coated nylon backs and hide-reinforced fingertips and back straps. Ideal for hospital andlaboratory cryogenic gloves with blue-coated nylon backs.

7 Pair*Please add suffix S for small or L for large to catalogue :Layout 1 17/6/10 16:47 Page 218219 Dewars and cryo accessoriesCeramic tweezersThese ceramic tweezers overcome the disadvantages of tweezers manufactured from conventionalmaterials such as stainless steel, titanium and plastic. They can be used for a variety of laboratoryand electronic applications due to their heat and corrosion resistance, insulation and anti-staticand non magnetic properties. Three shapes are available, straight pointed, curved or serrated, oneither a special aluminium alloy or polyacetal shank. The straight pointed tweezers are also availableon a stainless steel tweezers, straight, alloy shankT5151 Ceramic tweezers, curved, alloy shankT5152 Ceramic tweezers, serrated, alloy shankT5153 Ceramic tweezers, straight, polyacetal shankT5154 Ceramic tweezers, curved, polyacetal shankT5155 Ceramic tweezers, serrated, polyacetal shankT5156 Ceramic tweezers, straight, stainless steel shankESD cushion grip tweezersThese non-magnetic stainless steel precision tweezers with ESD safe red cushion grip handles havehigh resistivity.

8 They are particularly useful for tweezers, 115 mm, extra fine tips T5510 ESD tweezers, 120 mm, fine curved tips cryo pliersThese pliers are easy to use and have self-aligned copper bars for quick freezing. They are ideal forin situand in vivoapplications for both animal and plant tissue. Two models are available: one suitable for quick freezing micron-sized pieces of tissue; the other for millimetre-sized pliersT5371 cryo pliers for 1 mm tissueWarley. A. Cryofixation of heart tissue for X-ray microanalysis .Scanning Microscopy 3, 1247-1252. , and Buja, New techniques for the preparation of thin freeze-dried cryosections for X-ray microanalysis . , J-P., Barnard, T., Haggis, (eds), SEM Inc., AMF O'Hare, Chicago. The Science of Biological Specimen Preparation .T5153T5150T5154T5150T5152T5156T5509T551 09_Dewars_Cryo_0510:Layout 1 17/6/10 16:47 Page 219 Tel:+44 (0)1279 813519 Fax:+44 (0)1279 815106 and cryo accessoriesCryo grid boxesThese cryo grid boxes are used for transferring, storing and manipulating vitrified cryo TEMspecimens made with cryodevices like the FEI Vitrobot, Gatan 626 or Gatan CT3500cryotransfer systems and other cryovitrification processes.

9 There are two versions, each withfour storage positions. The round box is the most widely used and is available with orwithout a non-static rotatable lid. The square cryo TEM grid box includes a non-staticrotatable lid. All of the boxes have a 5/40 tap in the centre. On versions with lids, the lid isheld in place with a stainless steel screw. A handling rod is available for the cryo grid grid box with lid, roundG3728 cryo grid box base only, round G3735 cryo grid box with pin type lid, roundG3729 cryo grid box with lid, squareG3733 cryo grid box handling rodStainless steel freeze-drying holdersThis range of stainless steel, interlocking 25 mm sample holders is available for bulkspecimens and membrane filters. The material is plunged into the cryogen and transferredinto the pre-cooled sample holder. Using the handling tongs, the holders can be stackedonto a freeze-drying stage.

10 The holders allow safe handling of valuable low temperaturespecimens while minimising temperature freeze-drying sample holder, 25 mm diaG3937 Sample holder for membrane filter, 25 mm diaG3938 Handling tongsSpecimen pins for cryo -ultramicrotomyThese aluminium specimen mount pins are suitable for mounting samples for specimen pins. Pack of 50 cryo freezer hatsThese freezer hats for holding specimens in high pressure freezing equipment are availablein brass only. The planchette designs are illustrated. The depths of cavity are , mm. Using two planchettes, one on another, in various combinations, allows cavitythicknesses of , , , , and mm to be hats, type A. Pack of 50G3467 Freezer hats, type B. Pack of 50 Brass Planchette A mmBrass Planchette B 9_Dewars_Cryo_0510:Layout 1 17/6/10 16:47 Page 220221 Dewars and cryo accessoriesFro-Tissuer penThis pen is designed to prevent frozen sections from falling off, moving or wrinkling on the slide duringimmunostaining procedures.