ABSCIEX 4500 QTRAP 液相色谱质谱联用系统基础应用培训

1 ABSCIEX 4500 QTRAP ABSCIEX 3 2012 AB SCIEX QTRAP 4500 QTRAP 5500 Curtain Plate Orifice Qjet Q0 Q1 LINAC Q3 Detector 4 2012 AB SCIEX API ESI ABSCIEX TurboIonSpray TIS APCI ABSCIEX Heated Nebulizer HN APPI ABSCIEX PhotoSpray UV dopant 5 2012 AB SCIEX (IS) Orifice (DP) (CUR) Gas 2 (GS2) 750 C) Gas 1 (GS1) 2. + + + + + + + + - 3. + + + + + + + + - 1. + + + + + + + + - + 4. + + + 6 2012 AB SCIEX LC Flow +2-4kV 7 2012 AB SCIEX + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +.

2 8 2012 AB SCIEX : / 9 2012 AB SCIEX Turbo V TIS APCI mm mm 750 C APCI 10 2012 AB SCIEX / / 11 2012 AB SCIEX Turbo V TurboIonSpray ESI 6500 5500 5~100 L/min 200~600 L/min 800 L/min Curtain Gas CUR psi 15~20 35~40$ 40$ IonSpray Voltage IS V +5500 - 4500 +5500 - 4500 +5500 - 4500 Temperature TEM C 0 450~550 550 Ion Source Gas1 GS1 psi 15~20 40~55 55~70 Ion Source Gas2 GS2 psi 0 40~60 50~70 Collision Gas* CAD Medium Medium Medium *

3 $3200 4000 25 12 2012 AB SCIEX Orifice (DP) (CUR) (NC) Gas 1 (GS1) 1. 2. O2 N2 3. O2 N2 4. 5. O2 N2 13 2012 AB SCIEX 2000 Da 14 2012 AB SCIEX TurboIonSpray Heated Nebulizer 1. ESI APCI 2. 3. 1 2 3 15 2012 AB SCIEX Turbo V Heated Nebulizer APCI 600 L/min Curtain Gas CUR psi 40$ IonSpray Voltage IS V +5500 - 4500 Temperature TEM C 150~550 Ion Source Gas1 GS1 psi 40 Ion Source Gas2 GS2 psi N/A Interface Heater ihe On Collision Gas* CAD Medium * $3200 4000 25 16 2012 AB SCIEX Intensity, cps Mometasone Minoxidil TIS Time, min Intensity, cps Time, min Mometasone Minoxidil APCI 17 2012 AB SCIEX Turbo V SDS)

4 19 2012 AB SCIEX QTRAP 4500 QTRAP 5500 Curtain Plate Orifice Qjet Q0 20 2012 AB SCIEX Orifice Plate DP Qjet Q0 Curtain Plate Orifice Plate QJet XL Q0 21 2012 AB SCIEX 22 2012 AB SCIEX mTorr P QJet XL M+nHz M+xP+nHz=? x=1,2,3,.. 23 2012 AB SCIEX CUR Declustering Potential DP Orifice Plate DP DP CID 24 2012 AB SCIEX - 26 2012 AB SCIEX QTRAP 4500 QTRAP 5500 Q1 LINAC Q3 27 2012 AB SCIEX DC/RF + + + + 28 2012 AB SCIEX RF Filtering DC FDC U RF Rod Offset Voltage ROF RF only FDC RF ROF 29 2012 AB SCIEX 180 phase shift = B A B A RF RF RF RF 180 phase shift 180

5 Phase shift B A B A RF FDC 30 2012 AB SCIEX A A B B 31 2012 AB SCIEX FDC 32 2012 AB SCIEX ROV(IE) (IE) IE = , IE = , Q1 = n(Q0 - RO1);n Q1 IE1 =Q0-RO1= Q1 = n(RO2 - RO3); n Q3 IE3 =RO2-RO3= (1-6eV) 33 2012 AB SCIEX SIM SCAN 34 2012 AB SCIEX Analyst Q1 MS intensity 200 600 m/z intensity 200 600 m/z intensity 0 10 time Q3 MS, Q3 MS Center/Width, Q3 MI Q1 Q1 MS Center/Width Q1 MI 35 2012 AB SCIEX Q1 Q3 (MRM) 36 2012 AB SCIEX MRM Q1 Q2 Q3 37 2012 AB SCIEX MRM MRM MRM MRM MRM MRM 38 2012 AB SCIEX LINAC field on center line v field on center line v + v + v + 39 2012 AB SCIEX LINAC Q2 180 MRM

6 Q2 40 2012 AB SCIEX MRM ~300 MRM XIC of +MRM (297 pairs): amu from Sample 1 (MRMs 100) of Data MRM (Turbo Spray)Max. , n t e n s i t y , c p 2012 AB SCIEX QTRAP 4500 QTRAP 5500 Detector 43 2012 AB SCIEX 44 2012 AB SCIEX QTRAP 47 2012 AB SCIEX QTRAP MRM MRM& Ratio + = QTRAP 48 2012 AB SCIEX QTRAP 5500 QTRAP 4500 Q3/LIT Q3 LIT 1 ms 49 2012 AB SCIEX MRM SIM 6 (EPI) MS3 (EMS) (EMC)** (TDF)** 6 MRM-EPI MRM-MS3 -EPI -EPI EMS EPI.

7 >20 50 2012 AB SCIEX Q3/LIT 51 2012 AB SCIEX 52 2012 AB SCIEX QTRAP EMS Q2 2. LIT Q0 Q1 Q2 Q1 Q2 LIT RF only RF only CAD = high IQ3 EXB 53 2012 AB SCIEX QTRAP ER +/-15Da M+/- 15 amu RF only CAD = high Q1 Q2 Q1 Q2 LIT IQ3 EXB 54 2012 AB SCIEX QTRAP EPI RF only CAD = high Q0 Q1 Q2 Q1 Q2 LIT IQ3 EXB 2. Q2 55 2012 AB SCIEX QTRAP EPI Product Ion EPI Product Ion 56 2012 AB SCIEX QTRAP EPI vs. Product Ion EPI Product Ion 500 57 2012 AB SCIEX QTRAP EPI vs.

8 3D QTRAP 3D 58 2012 AB SCIEX QTRAP MS3 1 2 1 RF only CAD = high Q0 Q1 Q2 Q1 Q2 LIT IQ3 EXB 2 1 2. Q2 2 1 62 2012 AB SCIEX IDA Information Dependent Acquisition 64 2012 AB SCIEX Add to Exclusion List MRM NL, Prec, EMS Acquire MSMS Spectra IDA Criteria QTRAP 1: MRM NL, Prec, EMS 2: MRM 3: 2 <1ms EPI MRM MSMS 1 MRM-IDA-EPI 65 2012 AB SCIEX MRM-IDA-EPI MRM EPI MRM EPI EPI 66 2012 AB SCIEX QTRAP EPI MS2 QTRAP 67 2012 AB SCIEX Reserpine Q TRAP--Enhanced Product Ion Scan(EPI) QQQ-- Product Ion Scan MS2) > 500x QTRAP 68 2012 AB SCIEX