Acetone precipitation of proteins - Thermo Fisher Scientific
7. Allow the acetone to evaporate from the uncapped tube at room temperature for 30 minutes. Do not over-dry pellet, or it may not dissolve properly. 8. Add buffer appropriate for the downstream process and vortex thoroughly to dissolve protein pellet. TR0049.1 Acetone precipitation of proteins
Tags:
Acetone, Precipitation, Acetone precipitation
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document:
Documents from same domain
Acetone precipitation of proteins - Thermo Fisher Scientific
tools.thermofisher.com• Precipitation has an advantage over dialysis or desalting methods in that it enables concentration of the protein sample as well as purification from undesirable substances. • One disadvantage of protein precipitation is that proteins might denature, making the pellet difficult to re-solubilize.
Protein, Acetone, Precipitation, Acetone precipitation of proteins
Thermo Scienti˜ c TSQ Quantiva Triple-Stage Quadrupole ...
tools.thermofisher.comThermo Scienti˜ c TSQ Quantiva Triple-Stage Quadrupole Mass Spectrometer ... • Thermo Scientifi c ... New Zealand +64 9 980 6700 Norway +46 8 556 468 00 Russia/CIS +43 1 333 50 34 0 Singapore +65 6289 1190 Spain +34 914 845 965 Sweden +46 8 556 468 00 Switzerland +41 61 716 77 00
Ni-NTA Purification System - Thermo Fisher Scientific
tools.thermofisher.com7 Introduction Overview Introduction The Ni-NTA Purification System is designed for purification of 6xHis-tagged recombinant proteins expressed in bacteria, insect, and mammalian cells. The system is designed around the high affinity and selectivity of Ni-NTA Agarose
TRIzol Reagent User Guide - Pub. no. MAN0001271 - Rev. A
tools.thermofisher.comTRIzol™ Reagent Catalog Numbers 15596026 and 15596018 Doc. Part No. 15596026.PPS Pub. No. MAN0001271 Rev. A.0 WARNING! Read the Safety Data Sheets (SDSs) and follow the
Interpaion IEnPp fR nmpmeft n CfhRn Labware Chemical ...
tools.thermofisher.com286 www.thermoscientifi c.com Labwre Olafira nOPao ymreblo LsFoOrao LuapeDisEHLFbDabwriweFb CMT TTLy TL TM TT T/C T/ / ewFb TT na as H MnCM TM TTM Chemhical,l contaroAcSnlno#E N,o u u b u b b M M M b u u .
Interpretation of Nucleic Acid 260/280 Ratios
tools.thermofisher.comT123 – TECHNICAL BULLETIN NanoDrop Lite Interpretation of Nucleic Acid 260/280 Ratios T123– Rev 1/2012 Thermo Scientific NanoDrop Products Wilmington, Delaware USA Technical support:nanodrop.techsupport@thermofisher.com
Interpretation, Acid, Nucleic, Interpretation of nucleic acid 260
Corona CAD Charged Aerosol Detector - Thermo Fisher …
tools.thermofisher.com3 Corona CaD DeteCtor SpeCifiCationS* Operating Mode Charged Aerosol Detection Mobile Phase Flow Rate Up to 2 mL/min Full Scale Output Range 1 pA to 470 pA
GeneAmp PCR System 9700 - Thermo Fisher Scientific
tools.thermofisher.comIntroduction and Safety 1-1 Introduction and Safety 1 Overview About This Chapter This chapter provides information to help you safely operate the GeneAmp PCR System 9700. In This Chapter The following topics are covered in this chapter: Topics See Page About This Manual 1-2
alamarBlue TI read only - Thermo Fisher Scientific
tools.thermofisher.comPage 1 of 27 alamarBlue® Assay U.S. Patent No. 5,501,959 Indications for Use The alamarBlue® Assay is designed to measure quantitatively the proliferation of various human and animal cell lines, bacteria and fungi. The bioassay may also be used to establish relative
Installation Guide -Chromeleon 7 - Thermo Fisher Scientific
tools.thermofisher.comNote: Indicates information of special interest. Tip: Indicates information that will help you to use the software more efficiently. 1.3 Other Documents
Guide, Installation, Chromeleon, Installation guide chromeleon 7
Related documents
Immunohistochemistry (IHC)
images.novusbio.comprecipitation of soluble proteins, making alcohol-mediated fixation more appropriate for detection of membrane bound proteins. Aldehydes Alcohols ... Acetone fixation is generally mild and may be followed by fixation with alcohols or formaldehyde. Acetone.
SOL SOLSOLSOLUTIONSUTIONSUTIONS - NCERT
www.ncert.nic.inPrecipitation of substance ‘A’ ... Information :On adding acetone to methanol some of the hydrogen bonds between methanol molecules break. (i) At specific composition methanol-acetone mixture will form minimum boiling azeotrope …
How to Weld Type 2205 Code Plus Two Duplex Stainless Steel
www.titanmf.comTime-Temperature-Precipitation curve for a 2.25 inch (57 mm) thick 2205 plate with 0.14% nitrogen. The curve corresponds to an ambient temperature ... Common solvents such as acetone or mineral spirits can be used as cleaning agents. Moisture in the joint can cause porosity or weld metal cracking.
Gram Stain Protocols
asm.organd acetone solution), interacts with the lipids of the membranes of both gram-positive and gram-negative Bacteria. The outer membrane of the ... ammonium oxalate is added to prevent precipitation of the dye (McClelland, 2001) and uses an alcoholic solution of the counterstain.
I. Multiple Choice Questions (Type-I) 1. Which of the ...
cdn1.byjus.comPrecipitation of substance ‘A’ takes place when a small amount of ‘A’ is added to the solution. The solution is _____. (i) saturated (ii) supersaturated (iii) unsaturated (iv) concentrated . ... In ethanol and acetone mixture A–A or B–B type intermolecular .
Protein Sample Preparation - Bio-Rad
www.bio-rad.comlike Benzonase. Use protein precipitation with TCA/acetone (for example, with the ReadyPrep 2-D cleanup kit) to diminish carbohydrate content When a sample preparation protocol calls for a dilution, the two parts are stated like a ratio, but what is needed is a fraction. For example, “Dilute 1:2,” means to take 1 part
Acetone precipitation of proteins - Thermo Fisher Scientific
tools.thermofisher.com7. Allow the acetone to evaporate from the uncapped tube at room temperature for 30 minutes. Do not over-dry pellet, or it may not dissolve properly. 8. Add buffer appropriate for the downstream process and vortex thoroughly to dissolve protein pellet. TR0049.1 Acetone precipitation of proteins
Flame Atomic Absorption Spectrometry
www.agilent.comMinimize the amount of acetone which is carried over with the acetylene by: ‘Cracking’ the bottle before use by gently opening the valve to check for any drops or spray of acetone. Any bottle showing acetone should be returned to the supplier for replacement. Storing and using the bottles in the vertical position.
Experiment 7 — Nucleophilic Substitution
www.amherst.eduTo encourage an SN2 reaction mechanism you will use a solution of NaI in acetone. Iodide is a good nucleophile, and if it displaces bromide or chloride, NaBr or NaCl will precipitate (these are much less soluble in acetone than NaI). To encourage an SN1 reaction mechanism you will use a solution of AgNO3 in ethanol. Ethanol is a polar protic ...
TECHNIQUES FOR NANOPARTICLE SYNTHESIS
www.ijariit.com2.1 Chemical precipitation: It is a very simple method of synthesis of nanoparticles.The kinetics of nucleation and particle growth in homogeneous solutions can be adjusted by the controlled release of anions and cations. Careful control of precipitation kinetics can …