Transcription of Aerobic Gram-Negative Rods Flowchart - Centers for …
1 Aerobic Gram-Negative rods Flowchart gram Stain = gram negative rods or gram negative CoccobacilliGrowth on MacConkeyAgarYesLactose fermentation on MacLactose FermenterIndole test used for presumptive ID only Full Identification necessaryIndole(+)* Escherichia coli,Klebsiellaoxytoca,Citrobacter diversus,EdwardsiellatardaIndole (-)*Cronobacterspp. ,Klebsiellapneumoniae,Enterobacteraeroge nes, Enterobactercloacae, HafniaalveiLactose Non-fermenterOxidase Test used for presumptive ID only Full Identification necessaryOxidase (+)*Pseudomonas spp., Alcaligenesspp., Vibrio spp., Aeromonasspp., in/Rule out procedure for BT agents: Burkholderiamallei non-motile, Burkholderiapseudomallei motile, Both organisms are Indolenegative and ColistinresistantOxidase (-)*Acinetobacterspp.,Stenotrophomonassp p.,Proteus spp., Yersinia spp., Salmonella spp., Shigellaspp.,B. malleiIf probable B. mallei, use rule in/rule out procedure to leftNoGrowth on Blood AgarYesBrucellaspp.
2 , Francisellaspp., Eikenellaspp., Pasteurellaspp., CapnocytophagaRule in/Rule out procedure for BT agents: Francisellaspp: Oxidase negative , Catalase negative or weakly positive Brucellaspp: Oxidase positive, Urease positive, Catalase positiveNoGrowth on CHOCP robable Exceptions do exist to these general rules * = See Biochemical Tests for gram NegativeOrganism ID Job aid for positive and negativeresult job aid is a component of the free, on-demand CDC training course Biochemicals and gram negative Organism ID. Find the course at
