Transcription of Affinity of protein A and protein G to different IgG ...
1 Affinity of protein A and protein G to different IgG subclasses: Whole Antibodies protein A protein G protein A protein G. Human IgG1 ++ ++ Sheep IgG +/ ++. Human IgG2 ++ ++ Goat IgG +/ ++. Human IgG3 ++ Pig IgG ++ ++. Human IgG4 ++ ++ Chicken IgG +/ . Human IgA + . Human IgD + . Human IgE + Antibody Fragments Human IgM + protein A protein G. Mouse IgG1 + ++ Human Fab + +. Mouse IgG2a ++ ++ Human F(ab')2 + +. Mouse IgG2b ++ ++ Human scFv + . Mouse IgG3 + ++ Human Fc + +. Mouse IgM +/ Human . Rat IgG ++ ++ Human . Rat IgG1 +/ +. Rat IgG2a +/ ++. Rat IgG2b +/ +. Rat IgG2c +/ +. Rat IgM +/ . Rabbit IgG ++ ++ Key to affinities Hamster IgG + ++ ++ Strong Affinity Guinea Pig IgG ++ + + Moderate/slight Affinity Bovine IgG + ++ +/ Requires evaluation Horse IgG + ++ - No Affinity References Richman, D.
2 D., Cleveland, P. H., Oxman, M. N. and Johnson, K. M. 1982. The binding of 1. Staphylococci protein A by the sera of different animal species. J. Immunol. 128: 2300-2305. Frank, M. B. 1997. Antibody Binding to protein A and protein G beads . 5. In: Frank, M. B., ed. Molecular Biology Protocols. Oklahoma City.
