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Agilent 2100 Bioanalyzer System 2100 Expert Software …

Agilent TechnologiesAgilent 2100 Bioanalyzer System2100 Expert Software User's Guide2100 Expert Software User GuideNotices Agilent Technologies, Inc. 2000-2020No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or transla-tion into a foreign language) without prior agreement and written consent from Agi-lent Technologies, Inc. as governed by United States and international copyright NumberSD-UF0000050 Rev. in GermanyAgilent Technologies Hewlett-Packard-Strasse 8 76337 WaldbronnWarrantyThe material contained in this docu-ment is provided as is, and is sub-ject to being changed, without notice, in future editions.

1 Make sure a clean electrode cartridge is installed. 2 If you want to change the cartridge, follow the instructions in “Loading the Electrophoresis Chip into the 2100 Bioanalyzer Instrument” on …

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Transcription of Agilent 2100 Bioanalyzer System 2100 Expert Software …

1 Agilent TechnologiesAgilent 2100 Bioanalyzer System2100 Expert Software User's Guide2100 Expert Software User GuideNotices Agilent Technologies, Inc. 2000-2020No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or transla-tion into a foreign language) without prior agreement and written consent from Agi-lent Technologies, Inc. as governed by United States and international copyright NumberSD-UF0000050 Rev. in GermanyAgilent Technologies Hewlett-Packard-Strasse 8 76337 WaldbronnWarrantyThe material contained in this docu-ment is provided as is, and is sub-ject to being changed, without notice, in future editions.

2 Further, to the max-imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par-ticular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or perfor-mance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep-arate agreement shall Licenses The hardware and/or Software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such Rights LegendIf Software is for use in the performance of a Government prime contract or sub-contract.

3 Software is delivered and licensed as Commercial computer Software as defined in DFAR (June 1995), or as a commercial item as defined in FAR (a) or as Restricted computer Software as defined in FAR (June 1987) or any equivalent agency regu-lation or contract clause. Use, duplication or disclosure of Software is subject to Agi-lent Technologies standard commercial license terms, and non-DOD Departments and Agencies of the Government will receive no greater than Restricted Rights as defined in FAR (c)(1-2) (June 1987).

4 Government users will receive no greater than Limited Rights as defined in FAR (June 1987) or DFAR (b)(2) (November 1995), as applicable in any technical NoticesCAUTIONA CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per-formed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated condi-tions are fully understood and met. WARNINGA WARNING notice denotes a hazard.

5 It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indi-cated conditions are fully understood and Research Use OnlyNot for use in diagnostic procedures2100 Expert Software User Guide3In This This to the User s Guide for the Agilent 2100 Expert Software . This manual provides beginners and advanced users with information needed to successfully run electrophoretic assays with the 2100 Bioanalyzer System .

6 The 2100 Expert Software allows the control of the 2100 Bioanalyzer instrument (including diagnostic functions) and, in combination with an analysis kit, the acquisition, interpretation and result presentation of data generated during the analysis of DNA, RNA, and Typographic conventions in this Manual This chapter shows you how to make efficient use of this Start This chapter is meant for experienced users. It briefly summarizes the necessary steps to prepare and run an assay. Please note that in terms of the 2100 Expert Software , a method resembles an extended assay, which also includes additional administrative data such as operator, instrument, reporting, and workflow settings.

7 These additional functions are unlocked with the 2100 Expert Security Looking at 2100 Expert Software Before you start running methods/assays on the Agilent 2100 Bioanalyzer System , you should familiarize yourself with the 2100 Expert Software . This chapter shows how to get started with the 2100 Expert Software , and outlines its main operational Running and Evaluating Electrophoretic Methods/Assays This chapter explains how electrophoretic measurements are made using the 2100 Bioanalyzer System , gives detailed descriptions of all steps necessary to run electrophoretic assays.

8 And shows how to analyze and evaluate results using electropherograms and gel-like Expert Software User GuideIn This 5 Working with Chip Data and Methods/Assays This chapter shows you what to do to open, save, import and export files, and how to print the Administering System Functions and the Security Pack This chapter is your guideline for configuring the 2100 Expert Running Instrument Diagnostics This chapter shows how to use the diagnostic tests to check the 2100 Bioanalyzer instrument for proper Performing Verifications This chapter describes how you can validate your 2100 Bioanalyzer Products, Spare Parts.

9 And Accessories This chapter lists all parts and accessories including reorder Expert Software User Guide5 ContentsContents1 Typographic conventions in this Manual 72 Quick Start 9 Preparing the Agilent 2100 Bioanalyzer Instrument 10 Switching on the Agilent 2100 Bioanalyzer 11 Running a Measurement 12 During the chip run 20 Viewing the Measurement Results 21 What You Can do When the Measurement is Finished 233 Looking at 2100 Expert Software 25 Introduction to the Key Features of the 2100 Expert Software 26 Starting the 2100 Expert Software 272100 Expert Software Work Area 29 Closing 2100 Expert Software 37 The 2100 Expert Security Pack 384 Running and Evaluating Electrophoretic Methods/Assays 51 Principles of Nucleic Acid and Protein Analysis on a Chip 53 Preparing and Running an Electrophoretic Method or Assay 57 Analyzing and Evaluating the Results of an Electrophoretic Method or Assay 85 Result Flagging 1455 Working with Chip Data and

10 Methods/Assays 157 Working with Chip Data and Methods/Assays 1582100 Export Data Overview 159 Handling Methods 162 Handling Chip Data 166 Organizing, Backing up, and Archieving 2100 Expert Data 167 Importing Data 169 Exporting Data 173 Printing Reports 180 Configuring Tables 18762100 Expert Software User GuideContents 6 Administering System Functions and the Security Pack 191 Administering System Functions and the Security Pack 192 Access Control 193 Pre-configurations for the 2100 Expert Software 198 Configuring the 2100 Expert Software 207 Exporting and Importing Multiple Data Files 215 Archieving Data 217 Using Log Books


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